SHANK3 Antibodies
We offer SHANK3 Antibodies for use in common research applications: Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot. Each SHANK3 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our SHANK3 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the SHANK3 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our SHANK3 polyclonal antibodies and browse our SHANK3 monoclonal antibody catalog.
Alternate Names for SHANK3 Antibodies
anti-SHANK3 antibody, anti-DEL22q13.3 antibody, anti-KIAA1650proline rich synapse associated protein 2 antibody, anti-Proline-rich synapse-associated protein 2 antibody, anti-prosap2 antibody, anti-PSAP2SH3 and multiple ankyrin repeat domains protein 3 antibody, anti-SH3 and multiple ankyrin repeat domains 3 antibody, anti-shank postsynaptic density protein antibody, anti-SPANK-2 antibody
15 Results for "shank3" in Primary Antibodies
![Western Blot: SHANK3 Antibody (S69) [NBP1-47610] - Western Blot analysis of Rat brain membrane lysate showing detection of SHANK3 protein using Mouse Anti-SHANK3 Monoclonal Antibody, Clone S69-46 (NBP1-47610). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK3 Monoclonal Antibody (NBP1-47610) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/SHANK3-Antibody-S69-Western-Blot-NBP1-47610-img0005.jpg "Western Blot: SHANK3 Antibody (S69) [NBP1-47610] - Western Blot analysis of Rat brain membrane lysate showing detection of SHANK3 protein using Mouse Anti-SHANK3 Monoclonal Antibody, Clone S69-46 (NBP1-47610). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK3 Monoclonal Antibody (NBP1-47610) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: SHANK3 Antibody (S69) [NBP1-47610] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK3 Monoclonal Antibody, Clone S69-46 (NBP1-47610). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SHANK3 Monoclonal Antibody (NBP1-47610) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SHANK3 Antibody (D) Composite.](http://images.novusbio.com/fullsize/SHANK3-Antibody-S69-Immunocytochemistry-Immunofluorescence-NBP1-47610-img0007.jpg)
![Immunohistochemistry: SHANK3 Antibody (S69) [NBP1-47610] - Immunohistochemistry analysis using Mouse Anti-SHANK3 Monoclonal Antibody, Clone S69-46 (NBP1-47610). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-SHANK3 Monoclonal Antibody (NBP1-47610) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Early stages of filaggrin-like and dermal staining.](http://images.novusbio.com/fullsize/SHANK3-Antibody-S69-Immunohistochemistry-NBP1-47610-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: SHANK3 Antibody (S69) [NBP1-47610] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-SHANK3 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Borderline positive.](http://images.novusbio.com/fullsize/SHANK3-Antibody-S69-Immunocytochemistry-Immunofluorescence-NBP1-47610-img0003.jpg)
![Western Blot: SHANK3 Antibody [NBP2-41171] - Western blot analysis of SHANK3 in 3T3 cell lysate with SHANK3 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/SHANK3-Antibody-Western-Blot-NBP2-41171-img0001.jpg "Western Blot: SHANK3 Antibody [NBP2-41171] - Western blot analysis of SHANK3 in 3T3 cell lysate with SHANK3 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.")
![Immunohistochemistry: SHANK3 Antibody - BSA Free [NBP2-41171] - Immunohistochemistry of SHANK3 in human brain tissue with SHANK3 antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41171_rabbit-polyclonal-shank3-antibody-2711202420333840.jpg)
![Immunocytochemistry/ Immunofluorescence: SHANK3 Antibody - BSA Free [NBP2-41171] - Immunofluorescence of SHANK3 in human brain tissue with SHANK3 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41171_rabbit-polyclonal-shank3-antibody-271120241926181.jpg)
![Western Blot: SHANK3 Antibody (S367-51) [NBP2-42189] - Western Blot analysis of Monkey COS cells transfected with HA-tagged Shank1 showing detection of ~190 kDa SHANK1/SHANK3 protein using Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody, Clone S367-51 (NBP2-42189). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with HA-tagged Shank1. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody (NBP2-42189) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~190 kDa.](http://images.novusbio.com/fullsize/SHANK3-Antibody-S367-51-Western-Blot-NBP2-42189-img0003.jpg "Western Blot: SHANK3 Antibody (S367-51) [NBP2-42189] - Western Blot analysis of Monkey COS cells transfected with HA-tagged Shank1 showing detection of ~190 kDa SHANK1/SHANK3 protein using Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody, Clone S367-51 (NBP2-42189). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with HA-tagged Shank1. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody (NBP2-42189) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~190 kDa.")
![Immunocytochemistry/Immunofluorescence: SHANK3 Antibody (S367-51) [NBP2-42189] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody, Clone S367-51 (NBP2-42189). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody (NBP2-42189) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SHANK1/SHANK3 Antibody (D) Composite.](http://images.novusbio.com/fullsize/SHANK3-Antibody-S367-51-Immunocytochemistry-Immunofluorescence-NBP2-42189-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: SHANK3 Antibody (S367-51) [NBP2-42189] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X.](http://images.novusbio.com/fullsize/SHANK3-Antibody-S367-51-Immunocytochemistry-Immunofluorescence-NBP2-42189-img0002.jpg)
![Western Blot: SHANK3 Antibody [NBP3-13162] - Various tissue extracts (75 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Shank3 antibody (NBP3-13162) diluted at 1:1000.](http://images.novusbio.com/fullsize/SHANK3-Antibody-Western-Blot-NBP3-13162-img0001.jpg "Western Blot: SHANK3 Antibody [NBP3-13162] - Various tissue extracts (75 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Shank3 antibody (NBP3-13162) diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: SHANK3 Antibody [NBP3-13162] - SHANK3 antibody detects SHANK3 protein at cell body and synaptic vesicles by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: SHANK3 protein stained by SHANK3 antibody (NBP3-13162) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/SHANK3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13162-img0002.jpg)
![Immunohistochemistry-Frozen: SHANK3 Antibody [NBP3-13162] - SHANK3 antibody detects SHANK3 protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: SHANK3 protein stained by SHANK3 antibody (NBP3-13162) diluted at 1:250. Red: NeuN, stained by NeuN antibody [2Q158] diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/SHANK3-Antibody-Immunohistochemistry-Frozen-NBP3-13162-img0003.jpg)
![Western Blot: SHANK3 Antibody [NBP3-13167] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SHANK3 antibody (NBP3-13167) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/SHANK3-Antibody-Western-Blot-NBP3-13167-img0001.jpg "Western Blot: SHANK3 Antibody [NBP3-13167] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SHANK3 antibody (NBP3-13167) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: SHANK3 Antibody [NBP3-13167] - SHANK3 antibody detects SHANK3 protein by immunofluorescent analysis. Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: SHANK3 protein stained by SHANK3 antibody (NBP3-13167) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/SHANK3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13167-img0002.jpg)
![Western Blot: SHANK3 Antibody [NBP2-32161] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-SHANK3 antibody NBP2-32161 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/SHANK3-Antibody-Western-Blot-NBP2-32161-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: SHANK3 Antibody [NBP2-32161] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-SHANK3 antibody NBP2-32161 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: SHANK3 Antibody [NBP2-32161] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SHANK3 antibody NBP2-32161 used for IP at 6 ug/mg lysate. For blotting immunoprecipitated SHANK3, NBP2-32161 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/SHANK3-Antibody-Immunoprecipitation-NBP2-32161-img0002.jpg)