Neurexin 1 beta/NXRN1b Antibody

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Western blot shows lysates of rat spinal cord tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Rat Neurexin 1 beta /NXRN1b Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4524) followed by ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

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Neurexin 1 beta/NXRN1b Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant rat Neurexin 1 beta /NXRN1b
Ala47-Thr392
Accession # Q63373
Specificity
Detects rat Neurexin 1 beta /NXRN1b in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross‑reactivity with recombinant rat Neurexin 1 alpha is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
Nrxn1
Purity
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF4524 in the following application:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neurexin 1 beta/NXRN1b Antibody

  • Neurexin 1 beta
  • NXR1B
  • NXRN1b

Background

Neurexin 1 beta is an 80 kDa member of the Neurexin family of proteins. It is expressed on neuronal presynaptic membranes and contributes to synapse formation through binding to postsynaptic neuroligins. An alternate start site in the Neurexin 1 gene generate an alpha - (long) and beta - (short) isoform. Mature rat Neurexin 1 beta is a type I transmembrane glycoprotein that is 468 amino acids (aa) in length. It has a 346 aa extracellular region (aa 47‑392) that contains one laminin-like G domain (aa 91‑264). Within the G domain, the presence or absence of a splice site (aa 201‑230) materially impacts neuroligin binding. Over aa 47‑392, rat Neurexin 1 beta is 100% and 98% aa identical to mouse and human Neurexin 1 beta, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for Neurexin 1 beta/NXRN1b Antibody (AF4524) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using AF4524:
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Flow
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
Flow Rat 01/08/2015
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Summary

ApplicationFlow Cytometry
Sample TestedSee PMID 22840401
SpeciesRat

Blocking

Blocking DetailsSee PMID 22840401

Details

Detection NotesSee PMID 22840401

Comments

CommentsPublished: See PMID 22840401Independent review from antibodyresource.com

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Anonymous
01/08/2015
Application: Flow
Species: Rat

Bioinformatics

Gene Symbol Nrxn1
Entrez
Uniprot