Neuroligin 4X/NLGN4X Antibody [Unconjugated]

Images

 
Western blot shows lysates of human muscle tissue. PVDF membrane was probed with 1 µg/mL of Human Neuroligin 4/NLGN4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5158) followed by HRP-conjugated ...read more
Neuroligin 4/NLGN4 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Human Neuroligin 4/NLGN4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5158) at 5 µg/mL overnight ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Neuroligin 4X/NLGN4X Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Neuroligin 4/NLGN4
Met618-Thr673
Accession # Q8N0W4
Specificity
Detects human Neuroligin 4/NLGN4 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant human (rh) NLGN3 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
NLGN4X
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Control
Neuroligin 4X/NLGN4X Lysate (NBP2-64629)
Publications
Read Publication using
AF5158 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neuroligin 4X/NLGN4X Antibody [Unconjugated]

  • ASPGX2
  • AUTSX2
  • HLNX
  • HNL4X
  • HNLX
  • KIAA1260AUTSX2
  • MGC22376
  • neuroligin 4, X-linked
  • Neuroligin 4X
  • NL4
  • NLGN
  • NLGN4
  • NLGN4neuroligin 4
  • NLGN4X
  • X-linked

Background

Neuroligin 4 (NLGN4, NL4 or NL4X) is a 110 kDa type I transmembrane glycoprotein that is a member of the type B carboxyesterase/lipase family of proteins (1). Neuroligins are postsynaptically expressed on neurons and initiate excitatory presynapse maturation through binding to select isoforms of beta -neurexin (1‑3). The 816 amino acid (aa) human NLGN4 contains a 41 aa signal sequence, a 635 aa extracellular domain (ECD), a 21 aa transmembrane domain and a 119 aa cytoplasmic tail. The ECD possesses a nonfunctional esterase homology domain through which neuroligins, except for NLGN2, interact with neurexins (4). Human NLGN4 is found on the X‑chromosome. It shares 69%‑73% aa identity with NLGNs 1, 2 and 3, and 98% aa identity with NLGN4Y, a Y-chromosome-encoded neuroligin (1). Human NLGN4 ECD shares 62%, 99% and 99% aa identity with mouse, equine and canine NLGN4, respectively (5). Unlike other neuroligins, human NLGN4 does not appear to express alternate splice forms (1, 6). Crystalization of the NLGN4 ECD with and without beta ‑neurexin shows that NLGN4 forms a homodimer via a hydrophobic interface, but interactions with beta -neurexin are hydrophilic and calcium‑dependent (4, 6). NLGNs 3 and 4 bind syntrophin-gamma 2 intracellularly (7). Mutations of NLGN4 can be associated with rare cases of autism, Asperger or Tourette syndromes (8‑10). Mice with a loss-of-function mutation in NLGN4 show deficits in reciprocal social interactions and communication that are reminiscent of autism spectrum conditions in humans (11).

  1. Bollinger, M. F. et al. (2001) Biochem. J. 356:581.
  2. Chih, B. et al. (2005) Science 307:1324.
  3. Nam, C. I. and L. Chen (2005) Proc. Natl. Acad. Sci. USA 102:6137.
  4. Chen, X. et al. (2008) Nat. Struct. Mol. Biol. 15:50.
  5. Bollinger, M. F. et al. (2008) Proc. Natl. Acad. Sci. USA 105:6421.
  6. Fabrichny, I. P. et al. (2007) Neuron 56:979.
  7. Yamakawa H. et al. (2007) Biochem. Biophys. Res. Commun. 355:41.
  8. Jamain, S. et al. (2003) Nat. Genet. 34:27.
  9. Talebizadeh, Z. et al. (2006) J. Med. Genet. 43:e21.
  10. Lawson-Yuen, A. et al. (2008) Eur. J. Hum. Genet. 16:614.
  11. Jamain, S. et al. (2008) Proc. Natl. Acad. Sci. USA 105:1710.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Neuroligin 4X/NLGN4X Antibody (AF5158)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Video Protocols

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Bioinformatics

Gene Symbol NLGN4X
Uniprot