Neuroligin 4X/NLGN4X Antibody [Unconjugated]


Western blot shows lysates of NTera‑2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Neuroligin 4X/NLGN4X Antigen Affinity-purified Polyclonal more
Neuroligin 4/NLGN4 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Human Neuroligin 4/NLGN4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5158) at 5 µg/mL overnight more

Product Details

Reactivity HuSpecies Glossary
Applications WB, IHC

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Neuroligin 4X/NLGN4X Antibody [Unconjugated] Summary

E. coli-derived recombinant human Neuroligin 4/NLGN4
Accession # Q8N0W4
Detects human Neuroligin 4/NLGN4 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant human (rh) NLGN3 is observed.
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Read Publication using
AF5158 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neuroligin 4X/NLGN4X Antibody [Unconjugated]

  • ASPGX2
  • AUTSX2
  • HLNX
  • HNL4X
  • HNLX
  • KIAA1260AUTSX2
  • MGC22376
  • neuroligin 4, X-linked
  • Neuroligin 4X
  • NL4
  • NLGN
  • NLGN4
  • NLGN4neuroligin 4
  • NLGN4X
  • X-linked


Neuroligin 4 (NLGN4, NL4 or NL4X) is a 110 kDa type I transmembrane glycoprotein that is a member of the type B carboxyesterase/lipase family of proteins (1). Neuroligins are postsynaptically expressed on neurons and initiate excitatory presynapse maturation through binding to select isoforms of beta -neurexin (1‑3). The 816 amino acid (aa) human NLGN4 contains a 41 aa signal sequence, a 635 aa extracellular domain (ECD), a 21 aa transmembrane domain and a 119 aa cytoplasmic tail. The ECD possesses a nonfunctional esterase homology domain through which neuroligins, except for NLGN2, interact with neurexins (4). Human NLGN4 is found on the X‑chromosome. It shares 69%‑73% aa identity with NLGNs 1, 2 and 3, and 98% aa identity with NLGN4Y, a Y-chromosome-encoded neuroligin (1). Human NLGN4 ECD shares 62%, 99% and 99% aa identity with mouse, equine and canine NLGN4, respectively (5). Unlike other neuroligins, human NLGN4 does not appear to express alternate splice forms (1, 6). Crystalization of the NLGN4 ECD with and without beta ‑neurexin shows that NLGN4 forms a homodimer via a hydrophobic interface, but interactions with beta -neurexin are hydrophilic and calcium‑dependent (4, 6). NLGNs 3 and 4 bind syntrophin-gamma 2 intracellularly (7). Mutations of NLGN4 can be associated with rare cases of autism, Asperger or Tourette syndromes (8‑10). Mice with a loss-of-function mutation in NLGN4 show deficits in reciprocal social interactions and communication that are reminiscent of autism spectrum conditions in humans (11).

  1. Bollinger, M. F. et al. (2001) Biochem. J. 356:581.
  2. Chih, B. et al. (2005) Science 307:1324.
  3. Nam, C. I. and L. Chen (2005) Proc. Natl. Acad. Sci. USA 102:6137.
  4. Chen, X. et al. (2008) Nat. Struct. Mol. Biol. 15:50.
  5. Bollinger, M. F. et al. (2008) Proc. Natl. Acad. Sci. USA 105:6421.
  6. Fabrichny, I. P. et al. (2007) Neuron 56:979.
  7. Yamakawa H. et al. (2007) Biochem. Biophys. Res. Commun. 355:41.
  8. Jamain, S. et al. (2003) Nat. Genet. 34:27.
  9. Talebizadeh, Z. et al. (2006) J. Med. Genet. 43:e21.
  10. Lawson-Yuen, A. et al. (2008) Eur. J. Hum. Genet. 16:614.
  11. Jamain, S. et al. (2008) Proc. Natl. Acad. Sci. USA 105:1710.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Neuroligin 4X/NLGN4X Antibody (AF5158)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.

Filter By Application
Western Blot
All Applications
Filter By Species
All Species

Reviews for Neuroligin 4X/NLGN4X Antibody (AF5158) (0)

There are no reviews for Neuroligin 4X/NLGN4X Antibody (AF5158). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Neuroligin 4X/NLGN4X Antibody (AF5158) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
mFluor Violet Conjugated Antibodies

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Neuroligin 4X/NLGN4X Antibody [Unconjugated] and receive a gift card or discount.


Gene Symbol NLGN4X