Western blot shows lysates of human muscle tissue. PVDF membrane was probed with 1 µg/mL of Human Neuroligin 4/NLGN4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5158) followed by HRP-conjugated ...read more
Neuroligin 4/NLGN4 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Human Neuroligin 4/NLGN4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5158) at 5 µg/mL overnight ...read more
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Neuroligin 4X/NLGN4X Antibody [Unconjugated]
neuroligin 4, X-linked
Neuroligin 4 (NLGN4, NL4 or NL4X) is a 110 kDa type I transmembrane glycoprotein that is a member of the type B carboxyesterase/lipase family of proteins (1). Neuroligins are postsynaptically expressed on neurons and initiate excitatory presynapse maturation through binding to select isoforms of beta -neurexin (1‑3). The 816 amino acid (aa) human NLGN4 contains a 41 aa signal sequence, a 635 aa extracellular domain (ECD), a 21 aa transmembrane domain and a 119 aa cytoplasmic tail. The ECD possesses a nonfunctional esterase homology domain through which neuroligins, except for NLGN2, interact with neurexins (4). Human NLGN4 is found on the X‑chromosome. It shares 69%‑73% aa identity with NLGNs 1, 2 and 3, and 98% aa identity with NLGN4Y, a Y-chromosome-encoded neuroligin (1). Human NLGN4 ECD shares 62%, 99% and 99% aa identity with mouse, equine and canine NLGN4, respectively (5). Unlike other neuroligins, human NLGN4 does not appear to express alternate splice forms (1, 6). Crystalization of the NLGN4 ECD with and without beta ‑neurexin shows that NLGN4 forms a homodimer via a hydrophobic interface, but interactions with beta -neurexin are hydrophilic and calcium‑dependent (4, 6). NLGNs 3 and 4 bind syntrophin-gamma 2 intracellularly (7). Mutations of NLGN4 can be associated with rare cases of autism, Asperger or Tourette syndromes (8‑10). Mice with a loss-of-function mutation in NLGN4 show deficits in reciprocal social interactions and communication that are reminiscent of autism spectrum conditions in humans (11).
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Chen, X. et al. (2008) Nat. Struct. Mol. Biol. 15:50.
Bollinger, M. F. et al. (2008) Proc. Natl. Acad. Sci. USA 105:6421.
Fabrichny, I. P. et al. (2007) Neuron 56:979.
Yamakawa H. et al. (2007) Biochem. Biophys. Res. Commun. 355:41.
Jamain, S. et al. (2003) Nat. Genet. 34:27.
Talebizadeh, Z. et al. (2006) J. Med. Genet. 43:e21.
Lawson-Yuen, A. et al. (2008) Eur. J. Hum. Genet. 16:614.
Jamain, S. et al. (2008) Proc. Natl. Acad. Sci. USA 105:1710.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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