SHANK3 Antibody - Azide and BSA Free

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

SHANK3 Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit SHANK3 Antibody - Azide and BSA Free (NBP1-46768) is a polyclonal antibody validated for use in IHC and WB. Anti-SHANK3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide from human SHANK3 conjugated to blue carrier protein was used as the antigen.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SHANK3
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 10-50 ug/ml
  • Immunohistochemistry-Frozen 10-50 ug/ml
  • Immunohistochemistry-Paraffin 10-50 ug/ml
  • Western Blot 10-50 ug/ml
• Theoretical MW: 186 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

The antigen shares 97% identity with rat and mouse sequence.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Sterilized water
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Ammonium sulfate precipitation
• Reconstitution Instructions: Reconstitute 0.5 mg size in 0.5 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the 0.1 mg size is provided in reconstituted format.

Alternate Names for SHANK3 Antibody - Azide and BSA Free

• DEL22q13.3
• KIAA1650proline rich synapse associated protein 2
• Proline-rich synapse-associated protein 2
• prosap2
• PSAP2SH3 and multiple ankyrin repeat domains protein 3
• SH3 and multiple ankyrin repeat domains 3
• shank postsynaptic density protein
• Shank3
• SPANK-2

Background

Function: Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and Homer, respectively, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction. Subcellular location: Cytoplasm. Cell junction synapse. Cell junction synapse; postsynaptic cell membrane postsynaptic density. Note: Postsynaptic density of neuronal cells. Extends into the region subjacent to the PSD. Tissue specificity: Widely expressed in brain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SHANK3 Antibody (NBP1-46768)(2)

Publications using NBP1-46768

• Kawada K, Saito R, Mimori S et al. Regulation of neural differentiation and synaptogenic factors by silencing of ubiquitin ligase Dorfin Glob Drugs Therap 2017-12-27 (IB, Mouse)
• Zhang X, Li X, Wang H et al. Shank3 contributes to neuropathic pain by facilitating the SNI-dependent increase of HCN2 and the expression of PSD95 Neurosci. Res. 2020-05-23 [PMID: 32454040] (IHC-Fr, Rat)

Bioinformatics

• Gene Symbol: SHANK3
• Entrez:
  • 85358(Human)
  • 58234(Mouse)
  • 59312(Rat)
• Uniprot:
  • Q9BYB0(Human)