SF3A1 Antibody

Western Blot: SF3A1 Antibody [NB100-79843] - Detection of Human SF3a120/SAP114 on HeLa whole cell lysate using NB100-79843. SF3a120/SAP114 was also IPed by rabbit anti-SF3a120/SAP114 antibodies NB100-79844, NB100-79845, and NB100-79846.

Immunohistochemistry: SF3A1 Antibody [NB100-79843] - Sample: FFPE sections of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti- SF3a120/SAP114 used at a dilution of 1:200 (1 ug/ml). Detection: DAB

• Reactivity: Hu, Mu, Bv
• Applications: WB, IHC, IP, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

SF3A1 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 300 and 350 of human splicing factor 3a, subunit 1, 120kDa using the numbering given in entry NP_005868.1 (GeneID 10291).
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SF3A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Immunohistochemistry 1:200 - 1:2000
  • Immunohistochemistry-Paraffin 1:200 - 1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Use in chromatin immunoprecipitation reported in scientific literature (PMID 28504715).
• Control:

  SF3A1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SF3A1 Antibody

• pre-mRNA processing 21
• pre-mRNA splicing factor SF3a (120 kDa subunit)
• Prp21
• PRPF21
• SAP 114
• SAP114splicing factor 3a, subunit 1, 120kD
• SF3A120
• spliceosome associated protein 114
• Spliceosome-associated protein 114
• splicing factor 3 subunit 1
• splicing factor 3A subunit 1
• splicing factor 3a, subunit 1, 120kDa

Background

This gene encodes subunit 1 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 1 belongs to the SURP protein family; named for the SURP (also called SWAP or Suppressor-of-White-APricot) motifs that are thought to mediate RNA binding. Subunit 1 has tandemly repeated SURP motifs in its amino-terminal half while its carboxy-terminal half contains a proline-rich region and a ubiquitin-like domain. Binding studies with truncated subunit 1 derivatives demonstrated that the two SURP motifs are necessary for binding to subunit 3 while contacts with subunit 2 may occur through sequences carboxy-terminal to the SURP motifs. Alternative splicing results in multiple transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SF3A1 Antibody (NB100-79843)(3)

Publications using NB100-79843

• Han B, Park HK, Ching T et al. Human DBR1 modulates the recycling of snRNPs to affect alternative RNA splicing and contributes to the suppression of cancer development. Oncogene 2017-05-15 [PMID: 28504715] (Chemotaxis, WB, Human)
• Han B, Shen Y, Zhang P et al. Overlooked FANCD2 variant encodes a promising, portent tumor suppressor, and alternative polyadenylation contributes to its expression. Oncotarget 2017-04-04 [PMID: 28157704] (IP)
• Joo JH, Correia GP, Li JL et al. Transcriptomic Analysis of PNN- and ESRP1-regulated Alternative pre-mRNA Splicing in Human Corneal Epithelial Cells. Invest Ophthalmol Vis Sci 2013-01-08 [PMID: 23299472]

Review for SF3A1 Antibody (NB100-79843) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Panneerselvam Jayabal WB Human 11/07/2016

Summary

• Application: Western Blot
• Sample Tested: 20 ug whole cell lysate
• Species: Human

Bioinformatics

• Gene Symbol: SF3A1
• Entrez:
  • 10291(Human)
• Uniprot:
  • Q15459(Human)