SF3A1 Antibody

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Western Blot: SF3A1 Antibody [NB100-79843] - Detection of Human SF3a120/SAP114 on HeLa whole cell lysate using NB100-79843. SF3a120/SAP114 was also IPed by rabbit anti-SF3a120/SAP114 antibodies NB100-79844, ...read more
Immunocytochemistry/ Immunofluorescence: SF3A1 Antibody [NB100-79843] - Detection of Human SF3a120/SAP114 by IHC-IF. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-SF3a120/SAP114 ...read more
Immunohistochemistry-Paraffin: SF3A1 Antibody [NB100-79843] - Detection of Human SF3a120/SAP114 by Immunohistochemistry. Sample: FFPE section of human pancreatic islet cell tumor. Antibody: Affinity purified rabbit ...read more
Immunohistochemistry-Paraffin: SF3A1 Antibody [NB100-79843] - FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- SF3a120/SAP114 used at a dilution of 1:100. Detection: DAB staining using ...read more
Immunohistochemistry: SF3A1 Antibody [NB100-79843] - Sample: FFPE sections of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti- SF3a120/SAP114 used at a dilution of 1:200 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP, ChIP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

SF3A1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 300 and 350 of human splicing factor 3a, subunit 1, 120kDa using the numbering given in entry NP_005868.1 (GeneID 10291).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SF3A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Chromatin Immunoprecipitation (ChIP)
Application Notes
1:200 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Use in chromatin immunoprecipitation reported in scientific literature (PMID 28504715).
Positive Control
SF3A1 Lysate (NBL1-15874)
Reviewed Applications
Read 1 Review rated 4
using
NB100-79843 in the following applications:

Publications
Read Publications using
NB100-79843 in the following applications:

  • 1 publication
  • IP
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SF3A1 Antibody

  • pre-mRNA processing 21
  • pre-mRNA splicing factor SF3a (120 kDa subunit)
  • Prp21
  • PRPF21
  • SAP 114
  • SAP114splicing factor 3a, subunit 1, 120kD
  • SF3a120
  • spliceosome associated protein 114
  • Spliceosome-associated protein 114
  • splicing factor 3 subunit 1
  • splicing factor 3A subunit 1
  • splicing factor 3a, subunit 1, 120kDa

Background

This gene encodes subunit 1 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 1 belongs to the SURP protein family; named for the SURP (also called SWAP or Suppressor-of-White-APricot) motifs that are thought to mediate RNA binding. Subunit 1 has tandemly repeated SURP motifs in its amino-terminal half while its carboxy-terminal half contains a proline-rich region and a ubiquitin-like domain. Binding studies with truncated subunit 1 derivatives demonstrated that the two SURP motifs are necessary for binding to subunit 3 while contacts with subunit 2 may occur through sequences carboxy-terminal to the SURP motifs. Alternative splicing results in multiple transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SF3A1 Antibody (NB100-79843)(3)

Review for SF3A1 Antibody (NB100-79843) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB100-79843:
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WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Panneerselvam Jayabal
WB Human 11/07/2016
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Summary

ApplicationWestern Blot
Sample Tested20 ug whole cell lysate
SpeciesHuman

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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Panneerselvam Jayabal
11/07/2016
Application: WB
Species: Human

Bioinformatics

Gene Symbol SF3A1
Entrez
Uniprot