Intersectin 2 Antibody

Western Blot: Intersectin 2 Antibody [NBP3-03747] - Analysis of extracts of various cell lines, using Intersectin 2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry/Immunofluorescence: Intersectin 2 Antibody [NBP3-03747] - Analysis of U-2 OS cells using Intersectin 2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunocytochemistry/Immunofluorescence: Intersectin 2 Antibody [NBP3-03747] - Analysis of C6 cells using Intersectin 2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunocytochemistry/Immunofluorescence: Intersectin 2 Antibody [NBP3-03747] - Analysis of L929 cells using Intersectin 2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Intersectin 2 Antibody Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 500-620 of human Intersectin 2 (NP_006268.2).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ITSN2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 50% glycerol, pH7.3.
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for Intersectin 2 Antibody

• intersectin 2
• KIAA1256SH3 domain-containing protein 1B
• PRO2015
• SH3 domain protein 1B
• SH3P18-like WASP associated protein
• SH3P18SH3D1Bintersectin-2
• SWA
• SWAPSH3P18-like WASP-associated protein

Background

Intersectin-2 is a cytoplasmic adapter protein that may provide a link between the actin assembly machinery and the endocytic membrance traffic, as well a control the production of clathrin-coated vesicles in terms of their invagination of budding. Intersectin-2 has four isoforms, with lengths of 1697, 1670, 1249, and 1192 amino acids and weights of 193, 190, 141, and 135 kDa respectively. Studies have been conducted on diseases and disorders related to this protein including Wiskott-Aldrich syndrome, dermatitis, sarcoma, schizophrenia, spasticity, pancreatitis, and pancreatic cancer. Intersectin-2 has also been known to have interactions with BCCIP, PTN, TBL3, MAP4K3, and SEMA6A in pathways such as the regulation of CDC42 activity pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITSN2