SF-1/NR5A1/Steroidogenic Factor 1 Antibody (OTI1B4) - Azide and BSA Free

Western Blot: SF-1/NR5A1/Steroidogenic Factor 1 Antibody (OTI1B4) - Azide and BSA Free [NBP2-74149] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR5A1.

Immunohistochemistry: SF-1/NR5A1/Steroidogenic Factor 1 Antibody (OTI1B4) - Azide and BSA Free [NBP2-74149] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: SF-1/NR5A1/Steroidogenic Factor 1 Antibody (OTI1B4) - Azide and BSA Free [NBP2-74149] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: OTI1B4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

SF-1/NR5A1/Steroidogenic Factor 1 Antibody (OTI1B4) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse SF-1/NR5A1/Steroidogenic Factor 1 Antibody (OTI1B4) - Azide and BSA Free (NBP2-46247) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-262 of human NR5A1(NP_004950) produced in E.coli.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: NR5A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 51.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for SF-1/NR5A1/Steroidogenic Factor 1 Antibody (OTI1B4) - Azide and BSA Free

• AD4BP
• AD4BPSTF-1
• Adrenal 4-binding protein
• ELP
• FTZ1adrenal 4 binding protein
• FTZF1
• FTZF1nuclear receptor AdBP4
• Fushi tarazu factor homolog 1
• NR5A1
• Nuclear receptor subfamily 5 group A member 1
• nuclear receptor subfamily 5, group A, member 1
• SF1
• SF-1
• SF-1POF7
• SF1steroidogenic factor 1
• Steroid hormone receptor Ad4BP
• steroidogenic factor-1

Background

Steroidogenic factor 1 (SF-1), a NR5 Fushi Tarazu-Like Receptor, has been shown to affect sexual differentiation, steroid metabolism, and neural development. SF-1 binds as a monomer and regulates all steroidogenic p450 genes, Mullerian inhibiting substance, the gonadotrophins, the ACTH receptor, oxytocin, nuclear receptor DAX-1, and several other genes. Four alternatively spliced isoforms of SF-1 have been isolated in mice, and they differ at the N and C termini but are identical in the DNA-binding domain. SF-1 expression has been documented in human brain, adrenal, spleen, liver, heart, ovary, testis, and placenta. ESTs have been isolated from human tissue libraries, including cancerous adrenal and normal lung and testis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NR5A1