SF-1/NR5A1/Steroidogenic Factor 1 Antibody (OTI1B4)

Western Blot: SF-1/NR5A1/Steroidogenic Factor 1 Antibody (1B4) [NBP2-46247] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR5A1.

Immunohistochemistry: SF-1/NR5A1/Steroidogenic Factor 1 Antibody (1B4) [NBP2-46247] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: SF-1/NR5A1/Steroidogenic Factor 1 Antibody (1B4) [NBP2-46247] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: OTI1B4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

SF-1/NR5A1/Steroidogenic Factor 1 Antibody (OTI1B4) Summary

• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-262 of human NR5A1(NP_004950) produced in E.coli.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: NR5A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
• Theoretical MW: 51.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SF-1/NR5A1/Steroidogenic Factor 1 Antibody (OTI1B4)

• AD4BP
• AD4BPSTF-1
• Adrenal 4-binding protein
• ELP
• FTZ1adrenal 4 binding protein
• FTZF1
• FTZF1nuclear receptor AdBP4
• Fushi tarazu factor homolog 1
• NR5A1
• Nuclear receptor subfamily 5 group A member 1
• nuclear receptor subfamily 5, group A, member 1
• SF1
• SF-1
• SF-1POF7
• SF1steroidogenic factor 1
• Steroid hormone receptor Ad4BP
• steroidogenic factor-1

Background

Steroidogenic factor 1 (SF-1), a NR5 Fushi Tarazu-Like Receptor, has been shown to affect sexual differentiation, steroid metabolism, and neural development. SF-1 binds as a monomer and regulates all steroidogenic p450 genes, Mullerian inhibiting substance, the gonadotrophins, the ACTH receptor, oxytocin, nuclear receptor DAX-1, and several other genes. Four alternatively spliced isoforms of SF-1 have been isolated in mice, and they differ at the N and C termini but are identical in the DNA-binding domain. SF-1 expression has been documented in human brain, adrenal, spleen, liver, heart, ovary, testis, and placenta. ESTs have been isolated from human tissue libraries, including cancerous adrenal and normal lung and testis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SF-1/NR5A1/Steroidogenic Factor 1 Antibody (NBP2-46247)(1)

Publication using NBP2-46247

• Goktepe O, Onder GO, Cetindag E et al. The effect of different doses of nonylphenol on the blood-testicular barrier integrity, hormone level, and DNA damage in the testes of rats Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2023-05-09 [PMID: 37164249] (WB, Rat)
  
  Details:
  1:1000 WB dilution

Bioinformatics

• Gene Symbol: NR5A1