SF-1/NR5A1/Steroidogenic Factor 1 Antibody (NR5A1/4368R)

Immunohistochemistry-Paraffin: SF-1/NR5A1/Steroidogenic Factor 1 Antibody (NR5A1/4368R) [NBP3-13917] - Formalin-fixed, paraffin-embedded human adrenal cortical tumor stained with SF-1/NR5A1/Steroidogenic Factor 1 antibody (NR5A1/4368R). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.

Immunohistochemistry-Paraffin: SF-1/NR5A1/Steroidogenic Factor 1 Antibody (NR5A1/4368R) [NBP3-13917] - Formalin-fixed, paraffin-embedded human renal oncocytoma stained with SF-1/NR5A1/Steroidogenic Factor 1 antibody (NR5A1/4368R). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.

• Reactivity: Hu, Rt
• Applications: IHC
• Clone: NR5A1/4368R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

SF-1/NR5A1/Steroidogenic Factor 1 Antibody (NR5A1/4368R) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14291)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment of human SF-1/NR5A1/Steroidogenic Factor 1 protein (exact sequence is proprietary) (Uniprot: Q13285)
• Localization: Nuclear
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NR5A1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
• Theoretical MW: 53 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for SF-1/NR5A1/Steroidogenic Factor 1 Antibody (NR5A1/4368R)

• AD4BP
• AD4BPSTF-1
• Adrenal 4-binding protein
• ELP
• FTZ1adrenal 4 binding protein
• FTZF1
• FTZF1nuclear receptor AdBP4
• Fushi tarazu factor homolog 1
• NR5A1
• Nuclear receptor subfamily 5 group A member 1
• nuclear receptor subfamily 5, group A, member 1
• SF1
• SF-1
• SF-1POF7
• SF1steroidogenic factor 1
• Steroid hormone receptor Ad4BP
• steroidogenic factor-1

Background

Steroidogenic factor 1 (SF-1), a NR5 Fushi Tarazu-Like Receptor, has been shown to affect sexual differentiation, steroid metabolism, and neural development. SF-1 binds as a monomer and regulates all steroidogenic p450 genes, Mullerian inhibiting substance, the gonadotrophins, the ACTH receptor, oxytocin, nuclear receptor DAX-1, and several other genes. Four alternatively spliced isoforms of SF-1 have been isolated in mice, and they differ at the N and C termini but are identical in the DNA-binding domain. SF-1 expression has been documented in human brain, adrenal, spleen, liver, heart, ovary, testis, and placenta. ESTs have been isolated from human tissue libraries, including cancerous adrenal and normal lung and testis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NR5A1
• Uniprot:
  • Q13285()
  • Q13285()