SETD8 Antibody [Biotin]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF (-), IHC (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Biotin

SETD8 Antibody [Biotin] Summary

Immunogen
A synthetic peptide made to an internal portion of the human SETD8 protein (between residues 100-200) [UniProt Q9NQR1]
Localization
Nucleus. Chromosome. Note: Specifically localizes to mitotic chromosomes. Associates with silent chromatin on euchromatic arms. Not associated with constitutive heterochromatin.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SETD8
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
Application Notes
This SETD8 antibody is useful for Western Blot where a band is seen ~42 kDa representing SETD8 protein. This antibody is not applicable for Immunocytochemistry or Immunohistochemistry.
Readout System

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for SETD8 Antibody [Biotin]

  • H4-K20-HMTase SETD8
  • H4K20-specific histone methyltransferase splice variant Set8b
  • Histone-lysine N-methyltransferase SETD8
  • KMT5A SET domain-containing protein 8
  • KMT5A
  • Lysine N-methyltransferase 5A
  • PR/SET domain containing protein 8
  • PR/SET domain-containing protein 07
  • PR/SET07
  • PRSET7
  • PR-Set7EC 2.1.1.43
  • SET domain containing (lysine methyltransferase) 8
  • SET07SET8H4-K20-specific histone methyltransferase
  • SET8

Background

SETD8 (KMT5A, SET8, PR-Set7, SET domain containing (lysine methyltransferase) 8) is a methyltransferase that appears to be involved in cell cycle regulation; primarily during G2/M phase. A recent study has shown that KMT5A is regulated by phosphorylation at Ser 29 by the cyclin-dependent kinase 1/cyclinB complex from prophase through early anaphase following accumulation of H4K20me1. While the phosphorylation did not alter KMT5A methyltransferase activity, it did cause the removal of KMT5A from mitotic chromosomes. This study also showed that dephosphorylation of Ser 29 in KMT5A during late mitosis is vital to proper mitotic progression. If Ser 29 is not dephosphorylated at the proper time, a delay between metaphase and anaphase occurs (PMID: 20966048). Another study showed that KMT5A binds to the H4 N-terminal tail, consequently blocking the acetylation of lysine residues K5, K8 and K12 of histone H4 during G1, which can inhibit DNA replication (PMID: 18418072).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SETD8