CDC14 Antibody (472508) [Unconjugated]

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Raji human Burkitt's lymphoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat CDC14 Monoclonal Antibody (Catalog # MAB4457) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CDC14 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, C2C12 mouse myoblast cell line, H4-II-E-C3 rat hepatoma cell line, L6 rat myoblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat CDC14 Monoclonal Antibody (Catalog # MAB4457) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for CDC14 at approximately 50-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 472508
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CDC14 Antibody (472508) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human CDC14A
  Met15-Gly366
  Accession # Q9UNH5
• Specificity: Detects endogenous human, mouse and rat CDC14A in Western blots. In Western blots, this antibody does not cross‑react with recombinant human PTEN. Reactivity with CDC14B is not known.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDC14A
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CDC14 Antibody (472508) [Unconjugated]

• CDC14
• cdc14;CDC14A;DFNB105;DFNB32;DFNB35;Dual specificity protein phosphatase CDC14A;hCDC14
• CDC14A

Background

Cell Division Cycle 14 (CDC14) is a dual tyrosine and serine/threonine phosphatase. There are two CDC14 proteins, CDC14A with a molecular weight of 70 kDa, and CDC14B with a molecular weight of 53 kDa. CDC14A is primarily associated with the nucleolus, bound to Cfi1/Net1 during cell cycle interphase. During anaphase and telophase, CDC14 redistributes to the nucleoplasm and cytoplasm, where it plays a role in segregating and separating sister chromatids. Mutations that alter CDC14 function cause uneven distribution of chromosomes in dividing cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CDC14 Antibody (MAB4457)(1)

Publication using MAB4457

• Marquis M, Boulet S, Mathien S, Rousseau J, Thebault P, Daudelin J, Rooney J, Turgeon B, Beauchamp C, Meloche S, Labrecque N The non-classical MAP kinase ERK3 controls T cell activation. PLoS ONE, 2014-01-27;9(1):e86681. 2014-01-27 [PMID: 24475167] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: CDC14A
• Uniprot:
  • Q9UNH5()