SETD8 Antibody [Alexa Fluor® 750]

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF (-), IHC (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Alexa Fluor 750

SETD8 Antibody [Alexa Fluor® 750] Summary

• Immunogen: A synthetic peptide made to an internal portion of the human SETD8 protein (between residues 100-200) [UniProt Q9NQR1]
• Localization: Nucleus. Chromosome. Note: Specifically localizes to mitotic chromosomes. Associates with silent chromatin on euchromatic arms. Not associated with constitutive heterochromatin.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KMT5A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for SETD8 Antibody [Alexa Fluor® 750]

• H4-K20-HMTase KMT5A
• H4-K20-HMTase SETD8
• H4K20-specific histone methyltransferase splice variant Set8b
• Histone-lysine N-methyltransferase SETD8
• KMT5A SET domain-containing protein 8
• KMT5A
• Lysine N-methyltransferase 5A
• PR/SET domain containing protein 8
• PR/SET domain-containing protein 07
• PR/SET07
• PRSET7
• PR-Set7
• PR-Set7EC 2.1.1.43
• SET domain containing (lysine methyltransferase) 8
• SET07
• SET07SET8H4-K20-specific histone methyltransferase
• SET8
• SETD8

Background

Histone methyltransferase that specifically monomethylates 'Lys-20' of histone H4. H4 'Lys-20'monomethylation is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression.Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes.Required for cell proliferation, probably by contributing to the maintenance of proper higher order structure of DNAduring mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substratecompared to free histones

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KMT5A