Serpin G1/C1 Inhibitor Antibody [Unconjugated]

Western blot shows lysates of human lung tissue and human ovary tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Serpin G1/C1 Inhibitor Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2488) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Serpin G1/C1 Inhibitor at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of human lung tissue, loaded at 0.2 mg/mL. A specific band was detected for Serpin G1/C1 Inhibitor at approximately 162 kDa (as indicated) using 1 µg/mL of Goat Anti-Human Serpin G1/C1 Inhibitor Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2488) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Serpin G1/C1 Inhibitor Antibody [Unconjugated] Summary

• Immunogen: Human plasma-derived Serpin G1/C1 Inhibitor
• Specificity: Detects human Serpin G1/C1 Inhibitor in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) Serpin A1, rhSerpin A3, rhSerpin A4, rhSerpin A5, rhSerpin C1, rhSerpin F1, rhSerpin F2, rhSerpin I1, rhSerpin I2, recombinant mouse (rm) Serpin D1 and rmSerpin E2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SERPING1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Simple Western 1 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Serpin G1/C1 Inhibitor Antibody [Unconjugated]

• C1 Inhibitor
• C1IN
• C1INH
• C1NH
• HAE1
• HAE2
• Serpin G1
• SERPING1 serpin peptidase inhibitor, clade G (C1 inhibitor), member 1

Background

As a member of the serpin superfamily of serine protease inhibitors, Serpin G1/C1 inhibitor is the physiological inhibitor of activated C1r and C1s, two serine proteases involved in the classical complement pathway. In addition, it inhibits plasma kallikrein and coagulation factor XIIa, two serine proteases involved in the processing of kininogen to release bradykinin. Therefore, it plays an important role in regulating activation of both the complement and contact systems (1). Serpin G1 deficiency results in hereditary angioedema, which is characterized by recurrent episodes of localized angioedema of the skin, gastrointestinal mucosa or upper respiratory mucosa (2). The deduced amino acid sequence of human Serpin G1 precursor consists of 500 residues with a signal peptide. The mature protein of 478 amino acid residues is heavily glycosylated (1).

1. Davis, A.E. III et al. (1993) Methods Enzymol. 223:97.
2. Davis, A.E. III (2004) Drug News Perspect. 17:439.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Serpin G1/C1 Inhibitor Antibody (AF2488)(1)

Publication using AF2488

• Zakharova N, Artemenko E, Podoplelova N, Sveshnikova A, Demina I, Ataullakhanov F, Panteleev M Platelet surface-associated activation and secretion-mediated inhibition of coagulation factor XII. PLoS ONE, 2015-02-17;10(2):e0116665. 2015-02-17 [PMID: 25688860] (Immunoprecipitation, Western Blot, Human)

Bioinformatics

• Gene Symbol: SERPING1