Serpin B5/Maspin Antibody

Images

 
Western Blot: MASPIN Antibody [NB110-97377] - Detection of Maspin in HeLa whole cell extracts using NB110-97377.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Serpin B5/Maspin Antibody Summary

Immunogen
Synthetic peptide made to an internal portion of human MASPIN (within residues 100-200). [Swiss-Prot# P36952]
Localization
Secreted, extracellular space.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SERPINB5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/ml
Application Notes
This MASPIN antibody is useful for Western blot, where a doublet is seen at ~40 kDa. Does not work in Immunocytochemistry/Immunofluorescence.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Serpin B5/Maspin Lysate (NBL1-15848)
Raji Lysate (NB820-59466)
Control Peptide
Serpin B5/Maspin Peptide (NB110-97377PEP)

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Serpin B5/Maspin Antibody

  • Maspin
  • Peptidase inhibitor 5
  • PI5
  • PI-5
  • PI5protease inhibitor 5 (maspin)
  • serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5
  • Serpin B5
  • serpin peptidase inhibitor, clade B (ovalbumin), member 5

Background

MASPIN (mammary serine protease inhibitor; SERPINB5 or PI5), is a member of SERPIN superfamily (serine protease inhibitor/non-inhibitor) which also includes certain other proteins such as plasminogen activator inhibitors 1/2 and alpha1-antitrypsin. MASPIN is an extracellular matrix tumor supressor which blocks the proliferative, invasive, and metastatic properties of breast as well as prostate cancers. Because it does not undergo S (stressed) to R (relaxed) conformational transition characteristic of active SERPINS, MASPIN does not exhibit serine protease inhibitory activity. MASPIN expression is down-regulated in breast, prostate, gastric and melanoma cancers, however, this protein is overexpressed in certain other malignancies such as pancreatic, gallbladder, colorectal, and thyroid cancers which reflects that MASPIN may play different activities in different cell types. These observations may be explained by MASPIN's distinct subcellular localization - cytoplasmic, nuclear or both cytoplasmic-nuclear expression; by interactions with extracellular matrix; its structure and epigenetic modifications. MASPIN is also expressed in corneal epithelial tissue, wherein it controls the migration/adhesion of corneal stromal fibroblasts and myofibroblasts during corneal wound healing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-97377AF405
Alexa Fluor 488 Labeled NB110-97377AF488
Alexa Fluor 647 Labeled NB110-97377AF647
Alexa Fluor 700 Labeled NB110-97377AF700
Biotin Labeled NB110-97377B
DyLight 405 Labeled NB110-97377V
DyLight 488 Labeled NB110-97377G
DyLight 550 Labeled NB110-97377R
DyLight 650 Labeled NB110-97377C
FITC Labeled NB110-97377F
HRP Labeled NB110-97377H

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Bioinformatics

Gene Symbol SERPINB5
Entrez