Serpin B5/Maspin Antibody - BSA Free

Western Blot: Serpin B5/Maspin Antibody [NB110-97377] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and MASPIN/Serpin B5 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Rabbit Anti-Human MASPIN/Serpin B5 Polyclonal Antibody (Catalog # NB110-97377) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for MASPIN/Serpin B5 at approximately 40 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.

Western Blot: MASPIN Antibody [NB110-97377] - Detection of Maspin in HeLa whole cell extracts using NB110-97377.

• Reactivity: Hu
• Applications: WB, ICC/IF (-), Mycoplasma
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Serpin B5/Maspin Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Serpin B5/Maspin Antibody - BSA Free (NB110-97377) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide made to an internal portion of human MASPIN (within residues 100-200). [Swiss-Prot# P36952]
• Localization: Secreted, extracellular space.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SERPINB5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Knockdown Validated
  • Western Blot 0.5 ug/ml
• Application Notes: This MASPIN antibody is useful for Western blot, where a doublet is seen at ~40 kDa. Does not work in Immunocytochemistry/Immunofluorescence. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Serpin B5/Maspin Overexpression Lysate
  Raji Whole Cell Lysate

• Control Peptide:

  Serpin B5/Maspin Peptide (NB110-97377PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Serpin B5/Maspin Antibody - BSA Free

• Maspin
• Peptidase inhibitor 5
• PI5
• PI-5
• PI5protease inhibitor 5 (maspin)
• serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5
• Serpin B5
• serpin peptidase inhibitor, clade B (ovalbumin), member 5

Background

MASPIN (mammary serine protease inhibitor; SERPINB5 or PI5), is a member of SERPIN superfamily (serine protease inhibitor/non-inhibitor) which also includes certain other proteins such as plasminogen activator inhibitors 1/2 and alpha1-antitrypsin. MASPIN is an extracellular matrix tumor supressor which blocks the proliferative, invasive, and metastatic properties of breast as well as prostate cancers. Because it does not undergo S (stressed) to R (relaxed) conformational transition characteristic of active SERPINS, MASPIN does not exhibit serine protease inhibitory activity. MASPIN expression is down-regulated in breast, prostate, gastric and melanoma cancers, however, this protein is overexpressed in certain other malignancies such as pancreatic, gallbladder, colorectal, and thyroid cancers which reflects that MASPIN may play different activities in different cell types. These observations may be explained by MASPIN's distinct subcellular localization - cytoplasmic, nuclear or both cytoplasmic-nuclear expression; by interactions with extracellular matrix; its structure and epigenetic modifications. MASPIN is also expressed in corneal epithelial tissue, wherein it controls the migration/adhesion of corneal stromal fibroblasts and myofibroblasts during corneal wound healing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SERPINB5