SerpinB2 Antibody - BSA Free

Western Blot: SerpinB2 Antibody [NBP1-33188] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAI2 antibody diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: SerpinB2 Antibody [NBP1-33188] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PAI2 protein stained by PAI2 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Immunohistochemistry-Paraffin: SerpinB2 Antibody [NBP1-33188] - NCIN87 xenograft , using PAI-2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunoprecipitation: SerpinB2 Antibody [NBP1-33188] - Immunoprecipitation of PAI2 protein from U87-MG whole cell extracts using 5 ug of PAI2 antibody. Western blot analysis was performed using PAI2 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Western Blot: SerpinB2 Antibody [NBP1-33188] -Sample (30 μg of whole cell lysate)A: U87-MG10% SDS PAGEdiluted at 1:10000The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: SerpinB2 Antibody [NBP1-33188] -Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PAI2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SerpinB2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SerpinB2 Antibody - BSA Free (NBP1-33188) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-SerpinB2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human SerpinB2. The exact sequence is proprietary.
• Localization: Cytoplasm, Secreted, extracellular space
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SERPINB2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:20000
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rabbit (83%), Sheep (81%), Bovine (82%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for SerpinB2 Antibody - BSA Free

• HsT1201
• Monocyte Arg-serpin
• PAI
• PAI2
• PAI2Urokinase inhibitor
• Placental plasminogen activator inhibitor
• PLANH2
• PLANH2PAI-2
• plasminogen activator inhibitor 2
• plasminogen activator inhibitor, type II (arginine-serpin)
• serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 2
• Serpin B2
• serpin peptidase inhibitor, clade B (ovalbumin), member 2
• Urokinase Inhibitor

Background

Inhibits urokinase-type plasminogen activator. The monocyte derived PAI-2 is distinct from the endothelial cell-derived PAI-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SerpinB2 Antibody (NBP1-33188)(1)

Publication using NBP1-33188

• Y Ni, GH Wu, JJ Cai, R Zhang, Y Zheng, JQ Liu, XH Yang, X Yang, Y Shen, JM Lai, XM Ye, SJ Mo Tubule-mitophagic secretion of SerpinG1 reprograms macrophages to instruct anti-septic acute kidney injury efficacy of high-dose ascorbate mediated by NRF2 transactivation International journal of biological sciences, 2022-08-08;18(13):5168-5184. 2022-08-08 [PMID: 35982894]

Bioinformatics

• Gene Symbol: SERPINB2