Serpin B5/Maspin Antibody [DyLight 405]

• Reactivity: Hu
• Applications: WB, ICC/IF (-), Mycoplasma
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: DyLight 405

Serpin B5/Maspin Antibody [DyLight 405] Summary

• Immunogen: Synthetic peptide made to an internal portion of human MASPIN (within residues 100-200). [Swiss-Prot# P36952]
• Localization: Secreted, extracellular space.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SERPINB5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Knockdown Validated
  • Western Blot 0.5 ug/ml
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Serpin B5/Maspin Antibody [DyLight 405]

• Maspin
• Peptidase inhibitor 5
• PI5
• PI-5
• PI5protease inhibitor 5 (maspin)
• serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5
• Serpin B5
• serpin peptidase inhibitor, clade B (ovalbumin), member 5

Background

MASPIN (mammary serine protease inhibitor; SERPINB5 or PI5), is a member of SERPIN superfamily (serine protease inhibitor/non-inhibitor) which also includes certain other proteins such as plasminogen activator inhibitors 1/2 and alpha1-antitrypsin. MASPIN is an extracellular matrix tumor supressor which blocks the proliferative, invasive, and metastatic properties of breast as well as prostate cancers. Because it does not undergo S (stressed) to R (relaxed) conformational transition characteristic of active SERPINS, MASPIN does not exhibit serine protease inhibitory activity. MASPIN expression is down-regulated in breast, prostate, gastric and melanoma cancers, however, this protein is overexpressed in certain other malignancies such as pancreatic, gallbladder, colorectal, and thyroid cancers which reflects that MASPIN may play different activities in different cell types. These observations may be explained by MASPIN's distinct subcellular localization - cytoplasmic, nuclear or both cytoplasmic-nuclear expression; by interactions with extracellular matrix; its structure and epigenetic modifications. MASPIN is also expressed in corneal epithelial tissue, wherein it controls the migration/adhesion of corneal stromal fibroblasts and myofibroblasts during corneal wound healing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SERPINB5