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Serpin A3/alpha 1-Antichymotrypsin Antibody

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Western Blot: Serpin A3/alpha 1-Antichymotrypsin Antibody [NBP1-90295] - Analysis in human plasma.
Western Blot: Serpin A3/alpha 1-Antichymotrypsin Antibody [NBP1-90295] - Analysis in human plasma.
Immunohistochemistry-Paraffin: Serpin A3/alpha 1-Antichymotrypsin Antibody [NBP1-90295] - Staining of human cerebral cortex.
Independent Antibodies: Immunohistochemistry-Paraffin: Serpin A3/alpha 1-Antichymotrypsin Antibody [NBP1-90295] - Staining of human cerebral cortex, cervix, uterine, colon and liver using Anti-SERPINA3 antibody ...read more
Immunohistochemistry-Paraffin: Serpin A3/alpha 1-Antichymotrypsin Antibody [NBP1-90295] - Staining of human colon.
Immunohistochemistry-Paraffin: Serpin A3/alpha 1-Antichymotrypsin Antibody [NBP1-90295] - Staining of human liver.
Immunohistochemistry-Paraffin: Serpin A3/alpha 1-Antichymotrypsin Antibody [NBP1-90295] - Staining of human cervix, uterine.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

        

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Serpin A3/alpha 1-Antichymotrypsin Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: QDKMEEVEAMLLPETLKRWRDSLEFREIGELYLPKFSISRDYNLNDILLQLGIEEAFTSKADLSGITGARNLAVSQVVHKAVLDVFEEGTEASAATAVKITLLSALVETRTIVRFNRPFLMIIVPTDTQNIFFMSKVTNPKQEGAPHR
Specificity
Specificity of human Serpin A3/alpha 1-Antichymotrypsin antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SERPINA3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 0.04-0.4 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
Serpin A3/alpha 1-Antichymotrypsin Protein (NBP1-90295PEP)
Publications
Read Publications using NBP1-90295.

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23696371)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Serpin A3/alpha 1-Antichymotrypsin Antibody

  • AACTMGC88254
  • ACTGIG25
  • alpha 1-Antichymotrypsin
  • alpha-1-antichymotrypsin
  • antitrypsin), member 3
  • Cell growth-inhibiting gene 24/25 protein
  • growth-inhibiting protein 24
  • growth-inhibiting protein 25
  • member 3
  • serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase
  • serine (or cysteine) proteinase inhibitor, clade A, member 3
  • Serpin A3
  • serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-90309] - Analysis using Anti-SERPINA1 antibody NBP1-90309 (A) shows similar pattern to independent antibody NBP1-90308 (B).
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Western Blot: Carboxypeptidase B1/CPB1 Antibody [NBP2-15698] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Carboxypeptidase B antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
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Western Blot: Carboxypeptidase B1/CPB1 Antibody [NBP2-15698] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Carboxypeptidase B antibody [N3C3] diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
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Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE was detected in immersion fixed paraffin-embedded sections of human liver using anti-human mouse monoclonal antibody (Catalog # NB110-60531) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.
Western Blot: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE Antibody (WUE-4) [NB110-60531] - Detection of ApoE in human tissue lysate using NB110-60531. Lane 1: liver Lane 2: brain
Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE Antibody (WUE-4) [NB110-60531] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using ApoE antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.
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Immunohistochemistry: Coagulation Factor II/Thrombin Antibody [NBP1-58268] - Analysis of human liver after heat-induced Antigen retrieval. Antibody concentration 5 ug/ml.
Immunohistochemistry-Paraffin: Coagulation Factor II/Thrombin Antibody [NBP1-58268] - Human Placenta Tissue
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Western Blot: Vimentin Antibody [NB300-223] - Analysis of tissue and cell lysates. Antibody at 1:5000 in red. [1] protein standard (red), [2] rat whole brain lysate, [3] HeLa, [4] SH-SY5Y, [5] HEK293, and [6] NIH-3T3 cell lysates. NB300-223 binds to the vimentin protein showing a single band at ~50 kDa. The blot was simultaneously probed with mouse mAb to MAP2C/D, dilution 1:5000 in green, revealing multiple bands around 280 kDa that correspond to full length MAP2A/2B isotypes, and ~70 kDa bands which are MAP2C/D isotypes. MAP2 isotypes are seen only in extracts containing neuronal lineage cells.
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Immunohistochemistry-Paraffin: Puromycin-sensitive aminopeptidase/NPEPPS Antibody [NBP2-13669] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
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Western Blot: Albumin Antibody [NB600-41532] - Analysis using the HRP conjugate of NB600-41532. Detection of Albumin in whole mouse liver extracts. Image courtesy of anonymous customer product review.
Immunocytochemistry/Immunofluorescence: Albumin Antibody [NB600-41532] - NLRP12 downregulates multiple JNK activating pathways in hepatocytes. For checking the purity of primary hepatocytes, hepatocytes isolated from healthy WT and Nlrp12-/- mouse livers were immunostained with antibody for hepatocyte-specific marker Albumin and counterstained with DAPI. Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/40396), licensed under a CC-BY licence.
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Publications for Serpin A3/alpha 1-Antichymotrypsin Antibody (NBP1-90295)(2)

We have publications tested in 1 application: IHC.


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FAQs for Serpin A3/alpha 1-Antichymotrypsin Antibody (NBP1-90295). (Showing 1 - 1 of 1 FAQs).

  1. I am interested on Alpha 1 Antichymotrypsin Antibody (NBP1-90295,100ul). Could you tell me the concentration?
    • The concentration of this product is 0.2 mg/ml.

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Bioinformatics

Gene Symbol SERPINA3
Entrez
Uniprot