Recombinant Human Serpin A12 Protein

Product Discontinued

Recombinant Human Serpin A12 Protein Summary

• Description: Recombinant bioactive protein containing 415 amino acids for Human Serpin A12
  
  

  Source:E. coli
  Amino Acid Sequence:(Q8IW75)

• Preparation Method: Determined to be >98% pure by SDS-PAGE
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: SERPINA12
• Purity: >98%, by SDS-PAGE
• Endotoxin Note: <0.1 ng/ug

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Application Notes: Unit Definition: > 1 unit/ug. One unit of Turbo3C (HRV3C) Protease cleaves >95% of 100ug of target protein at 4C for 16 hours
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 50 mM Tris, pH 7.5 + 150 mM NaCl.
• Concentration: Lyoph
• Purity: >98%, by SDS-PAGE
• Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex.

Alternate Names for Recombinant Human Serpin A12 Protein

• antitrypsin), member 12
• member 12
• OL-64
• OL-64Visceral adipose-specific serpin
• serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase
• Serpin A12
• serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin)
• Vaspin
• Visceral adipose tissue-derived serine protease inhibitor

Background

Vaspin (visceral adipose-specific SERPIN), a newly identified adipokine, is a member of serine protease inhibitor family. Vaspin is also a unique insulin sensitizing adipocytokine in obesity. A recent publication indicates that induction of human vaspin mRNA expression in adipose tissue is regulated in a fat depot-specific manner and could be associated with parameters of obesity, insulin resistance, and glucose metabolism. Recombinant human Vaspin produced in E.Coli is a single, non-glycosylated polypeptide chain containing 415 amino acids and having a molecular mass of 47 kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: SERPINA12