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Human, Mouse, Rat Serpin A12 ELISA Kit (Colorimetric)

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Product Details

Summary
Product Discontinued
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    • Catalog Number
      KA1688
    • Availability
      Product Discontinued

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Human, Mouse, Rat Serpin A12 ELISA Kit (Colorimetric) Summary

Description
SERPINA12/Serpina12 (Human Mouse Rat) ELISA Kit is an in vitro quantitative assay for detecting SERPINA12/Serpina12 based on the principle of Competitive Enzyme Immunoassay.
Standard Curve Range
1 to 10000 pg/mL
Sensitivity
26.2 pg/mL
Assay Type
Competitive ELISA
Inter-Assay
CV% < 15%
Intra-Assay
CV% < 10%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
SERPINA12

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
Useful for quantification.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Anti-Vaspin polyclonal antibody (Item N)
  2. Assay Diluent A (Item D): contains 0.09% sodium azide as preservative.Diluent for Standards and serum or plasmas.
  3. Assay Diluent B (Item E): 5x concentrated buffer. Diluent for Standardsand cell culture media or other sample types.
  4. Biotinylated Vaspin peptide (Item F)
  5. HRP-conjugated Streptavidin.600 µlPositive control (Item M)
  6. HRP-Streptavidin concentrate (Item G) 500x concentratedHRP-conjugated Streptavidin.
  7. Standard Vaspin Peptide (Item C)
  8. Stop Solution (Item I): 0.2 M sulfuric acid.
  9. TMB One-Step Substrate Reagent (Item H): 3, 3’, 5, 5’-tetramethylbenzidine (TMB) in buffered solution.
  10. Vaspin Microplate (Item A) coated with secondary antibody.
  11. Wash Buffer Concentrate (20x) (Item B)

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human, Mouse, Rat Serpin A12 ELISA Kit (Colorimetric)

  • antitrypsin), member 12
  • member 12
  • OL-64
  • OL-64Visceral adipose-specific serpin
  • serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase
  • Serpin A12
  • serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin)
  • Vaspin
  • Visceral adipose tissue-derived serine protease inhibitor

Background

SERPINA12 may modulates insulin action conceivably only in the presence of its yet undefined target proteases in whiteadipose tissues

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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FAQs for Serpin A12 ELISA Kit (KA1688). (Showing 1 - 1 of 1 FAQ).

  1. What is the difference between the Quantikine and Duoset ELISA kits?
    • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

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Bioinformatics

Gene Symbol SERPINA12