Human Serpin A1/alpha 1-Antitrypsin ELISA Kit (Colorimetric)

ELISA: Serpin A1/alpha 1-Antitrypsin ELISA Kit [Biotin] [KA0459] - Standard curve

• Reactivity: Hu
• Applications: ELISA, NULL
• Suitable Sample Type: Cell Culture Supernatant, Cell Lysate, CSF, Milk, Plasma, Saliva, Serum, Urine
• Standard Curve Range: 0.391 to 25 ng/mL
• Sensitivity: 0.31 ng/mL

Human Serpin A1/alpha 1-Antitrypsin ELISA Kit (Colorimetric) Summary

• Description: SERPINA1 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human SERPINA1.
• Standard Curve Range: 0.391 to 25 ng/mL
• Sensitivity: 0.31 ng/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 7.10%
• Intra-Assay: CV% < 4.10%
• Spike Recovery: 98.50%
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: SERPINA1

Applications/Dilutions

• Dilutions:
  • ELISA
  • Quantification

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human Serpin A1/alpha 1-Antitrypsin ELISA Kit (Colorimetric)

• A1A
• A1AT
• AATMGC23330
• alpha 1-Antitrypsin
• alpha 1-Proteinase Inhibitor
• Alpha-1 protease inhibitor
• Alpha-1-antiproteinase
• alpha-1-antitrypsin
• antitrypsin), member 1
• member 1
• PIMGC9222
• serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase
• serine (or cysteine) proteinase inhibitor, clade A, member 1
• Serpin A1
• serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin)

Background

The SERPINA1 (Human) ELISA Kit is designed for detection of A1AT in human urine, milk, saliva, CSF and cell culture samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures A1AT in less than 4 hours. A polyclonal antibody specific for A1AT has been pre-coated onto a 96-well microplate with removable strips. Human A1AT in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for A1AT, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Serpin A1/alpha 1-Antitrypsin ELISA Kit (KA0459)(4)

Publications using KA0459

• Jun B, Jia-Yong Z, Wang L et al. iTRAQ-based proteomic analysis reveals potential regulatory networks in dust mite-related asthma treated with subcutaneous allergen immunotherapy. Mol Med Rep. 2020-09-01 [PMID: 32901873]
• Strange C, Stoller JK, Sandhaus RA et al. Results of a survey of patients with alpha-1 antitrypsin deficiency. Respiration 2005-08-03 [PMID: 16141711]
• Abboud RT, Ford GT, Chapman KR. et al. Emphysema in alpha1-antitrypsin deficiency: does replacement therapy affect outcome? Treat Respir Med 2005-01-01 [PMID: 15725045]
• Chappell S, Guetta-Baranes T, Batowski K et al. Haplotypes of the alpha-1 antitrypsin gene in healthy controls and Z deficiency patients. Hum Mutat 2004-12-01 [PMID: 15532029]

FAQs for Serpin A1/alpha 1-Antitrypsin ELISA Kit (KA0459). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: SERPINA1