Human Serpin A1/alpha 1-Antitrypsin ELISA Kit (Colorimetric)

ELISA: Serpin A1/alpha 1-Antitrypsin ELISA Kit [Biotin] [KA0459] - Standard curve

• Reactivity: Hu
• Applications: ELISA, NULL
• Suitable Sample Type: Cell Culture Supernatant, Cell Lysate, CSF, Milk, Plasma, Saliva, Serum, Urine
• Standard Curve Range: 0.391 to 25 ng/mL
• Sensitivity: 0.31 ng/mL

Human Serpin A1/alpha 1-Antitrypsin ELISA Kit (Colorimetric) Summary

• Description: SERPINA1 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human SERPINA1.
• Standard Curve Range: 0.391 to 25 ng/mL
• Sensitivity: 0.31 ng/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 7.10%
• Intra-Assay: CV% < 4.10%
• Spike Recovery: 98.50%
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: SERPINA1

Applications/Dilutions

• Dilutions:
  • ELISA
  • Quantification

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human Serpin A1/alpha 1-Antitrypsin ELISA Kit (Colorimetric)

• A1A
• A1AT
• AATMGC23330
• alpha 1-Antitrypsin
• alpha 1-Proteinase Inhibitor
• Alpha-1 protease inhibitor
• Alpha-1-antiproteinase
• alpha-1-antitrypsin
• antitrypsin), member 1
• member 1
• PIMGC9222
• serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase
• serine (or cysteine) proteinase inhibitor, clade A, member 1
• Serpin A1
• serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin)

Background

The SERPINA1 (Human) ELISA Kit is designed for detection of A1AT in human urine, milk, saliva, CSF and cell culture samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures A1AT in less than 4 hours. A polyclonal antibody specific for A1AT has been pre-coated onto a 96-well microplate with removable strips. Human A1AT in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for A1AT, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Serpin A1/alpha 1-Antitrypsin ELISA Kit (KA0459)(4)

Publications using KA0459

• Jun B, Jia-Yong Z, Wang L et al. iTRAQ-based proteomic analysis reveals potential regulatory networks in dust mite-related asthma treated with subcutaneous allergen immunotherapy. Mol Med Rep. 2020-09-01 [PMID: 32901873]
• Strange C, Stoller JK, Sandhaus RA et al. Results of a survey of patients with alpha-1 antitrypsin deficiency. Respiration 2005-08-03 [PMID: 16141711]
• Abboud RT, Ford GT, Chapman KR. et al. Emphysema in alpha1-antitrypsin deficiency: does replacement therapy affect outcome? Treat Respir Med 2005-01-01 [PMID: 15725045]
• Chappell S, Guetta-Baranes T, Batowski K et al. Haplotypes of the alpha-1 antitrypsin gene in healthy controls and Z deficiency patients. Hum Mutat 2004-12-01 [PMID: 15532029]

Bioinformatics

• Gene Symbol: SERPINA1