Semaphorin 6A Antibody

• Reactivity: Hu, Mu, Rt, Ch, Xp
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Semaphorin 6A Antibody Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: Semaphorin 6A synthetic peptide (coupled to carrier protein) corresponding to amino acids in the cytoplasmic region of human Sema-6A. The sequence used has high homology to similar regions in rat and mouse Sema-6A, and has low homology to other semaphorin family members. [UniProt# Q9H2E6]
• Specificity: The antibody detects a 120kDa protein corresponding to the apparent molecular mass ofSemaphorin 6A on SDS-PAGE immunoblots of Sema-6A transfected COS7 cells, human colorectal cancer cells, and rat PC12 cells. In addition, shRNA knockdown of Sema-6A expression significantly reduces detection of Sema-6A using anti-Sema-6A (a.a. 772-787) antibody.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SEMA6A
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:250
  • Immunoprecipitation 1:100
  • Western Blot 1:1000
• Theoretical MW: 120 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for Semaphorin 6A Antibody

• KIAA1368HT018
• sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A
• Sema VIA
• SEMA
• Sema6A
• SEMA6A1
• SEMA6A-1
• Semaphorin 6A
• semaphorin 6A-1
• Semaphorin VIA
• semaphorin-6A
• semaphorin-6A-1
• SEMAQ
• VIA

Background

Can act as repulsive axon guidance cues. May play a role in channeling sympathetic axons into the sympathetic chains and controlling the temporal sequence of sympathetic target innervation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SEMA6A