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Plexin A4 Antibody

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 (1 Review)
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Immunohistochemistry-Paraffin: Plexin A4 Antibody [NBP1-85128] - Staining of human cerebellum shows cytoplasmic positivity in Purkinje cells.
Immunohistochemistry-Paraffin: Plexin A4 Antibody [NBP1-85128] - Staining of human cerebellum shows cytoplasmic positivity in Purkinje cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Plexin A4 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SHVKVAGVECSPLVDGYIPAEQIVCEMGEAKPSQHAGFVEICVAVCRPEFMARSSQLYYFMTLTLSDLKPSRGPMSGGTQVTI
Specificity
Specificity of human Plexin A4 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Predicted Species
Rat (96%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PLXNA4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
Plexin A4 Protein (NBP1-85128PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-85128 in the following applications:
Publications
Read Publications using
NBP1-85128 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30735130).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Plexin A4 Antibody

  • A
  • DKFZp434G0625
  • FAYV2820
  • FLJ35026
  • FLJ38287
  • KIAA1550DKFZp566O0546
  • PLEXA4
  • Plexin A4
  • plexin A4, B
  • plexin-A4
  • PLXNA4
  • PLXNA4A
  • PLXNA4B
  • PRO34003

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Simple Western lane view shows lysates of bEnd.3 mouse endothelioma cell line, loaded at 0.2 mg/mL. A specific band was detected for Plexin A2 at approximately 246 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat Plexin A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5486) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the66-440 kDa separation system.
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Western blot shows lysates of mouse embryonic heart tissue. PVDF Membrane was probed with 1 µg/mL of Mouse Plexin A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4309) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Plexin A1 at approximately 200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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SLC35A1 GST (N-Term) ...
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[Unconjugated]
1250-S3
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(2 Reviews)
(27 Publications)
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Publications for Plexin A4 Antibody (NBP1-85128)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC-P.


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IHC-P
(1)
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Review for Plexin A4 Antibody (NBP1-85128) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-85128:
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(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Plexin A4 NBP1-85128
Enlarge 		5
reviewed by:
Anonymous
 IHC-Fr Mouse 04/25/2013
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Testedmouse brain
SpeciesMouse
LotR30914
CommentsI also tried at 1:200 and works at that dilution.

Blocking

Blocking Details30 minutes with Normal Goat Serum, 3% BSA, and 0.01% Triton-X in 1x PBS (PBST)

Primary Anitbody

Dilution Ratio1:100 at 4degreesC in a humidified chamber, overnight in PBST

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG, Alexa 568 (Life Technologies)
Secondary Manufacturer Cat#A11011
Secondary Concentration1:400

Details

Detection NotesImmunoflourescence, Zeiss Axio Imager
Fixation DetailsAntigen Retrieval, Sodium Citrate pH 6.0
Wash Description3x for 8 minutes in 1x PBS

Comments

CommentsI also tried at 1:200 and works at that dilution.

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FAQs for Plexin A4 Antibody (NBP1-85128) (0)

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Secondary Antibodies

 

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Immunohistochemistry-Frozen Plexin A4 NBP1-85128
Enlarge
 Anonymous
04/25/2013
Application: IHC-Fr
Species: Mouse
read this review

Bioinformatics

Gene Symbol PLXNA4