Semaphorin 6A Antibody [Unconjugated]

• Reactivity: Hu
• Applications: WB, Flow, IHC, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Semaphorin 6A Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Semaphorin 6A
  Gly19-Thr649
  Accession # Q9H2E6
• Specificity: Detects human Semaphorin 6A in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse Semaphorin 6A is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SEMA6A
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Semaphorin 6A Antibody [Unconjugated]

• KIAA1368HT018
• sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A
• Sema VIA
• SEMA
• Sema6A
• SEMA6A1
• SEMA6A-1
• Semaphorin 6A
• semaphorin 6A-1
• Semaphorin VIA
• semaphorin-6A
• semaphorin-6A-1
• SEMAQ
• VIA

Background

The semaphorins constitute a large family of secreted, glycosylphosphatidylinositol (GPI)-anchored and transmembrane cell signaling molecules. Depending on their domain organization and species origin, these proteins can be classified into eight groups. To date, at least 19 vertebrate Semaphorins belonging to five groups (class 3 to 7), have been identified. All Semaphorins contain a conserved 500 amino acid (aa) Sema domain at the amino-terminus. Semaphorins are best known for their roles in axon guidance during neuronal development. They are also expressed in non-neuronal tissues and are involved in angiogenesis, hematopoiesis, organogenesis, and the regulation of immune functions (1, 2). Class 6 Semaphorins (Sema 6) are transmembrane proteins that share homology with the axon-guiding insect Sema 1A. Human Sema 6A (VIa) cDNA predicts a 1,030 aa protein comprised of an extracellular domain, a transmembrane domain, and a long cytoplasmic tail (3, 4). A secreted form of Sema 6A can repel sympathetic and dorsal root ganglion axons in vitro, indicating a traditional role as an axon guidance signal (5). There is evidence, however, that Sema 6A also functions as a guidance receptor. Sema 6A mutants show a defect in thalamocortical neuron projection that is cell autonomous, and the cytoplasmic tails for Sema 6 contain binding sites for intracellular regulatory molecules such as Evl and Src (6).

1. Fiore, R. and A.W. Puschel (2003) Frontiers Biosci. 8:484.
2. Goshima, Y. et al. (2002) J. Clin. Invest. 109:993.
3. Zhou, et al. (1997) Mol. Cell Neurosci. 9:26.
4. Kikuchi, K. et al. (1999) Mol. Cell Neurosci. 13:9.
5. Xu, X-M. et al. (2000) J. Neurosci. 20:2638.
6. Leighton, P.A. et al. (2001) Nature 410:174.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Semaphorin 6A Antibody (AF1146)(4)

Publications using AF1146

• Y Sun, Y Xiao, H Sun, Z Zhao, J Zhu, L Zhang, J Dong, T Han, Q Jing, J Zhou, Z Jing miR-27a regulates vascular remodeling by targeting endothelial cells' apoptosis and interaction with vascular smooth muscle cells in aortic dissection Theranostics, 2019;9(25):7961-7975. 2019 [PMID: 31695809] (Western Blot, Human)
• S Demolli, A Doddaballa, K Devraj, K Stark, Y Manavski, A Eckart, CM Zehendner, T Lucas, T Korff, M Hecker, S Massberg, S Liebner, D Kaluza, RA Boon, S Dimmeler Shear stress-regulated miR-27b controls pericyte recruitment by repressing SEMA6A and SEMA6D Cardiovasc. Res., 2017;113(6):681-691. 2017 [PMID: 28453731] (Western Blot, Human)
• Urbich C, Kaluza D, Fromel T, Knau A, Bennewitz K, Boon RA, Bonauer A, Doebele C, Boeckel JN, Hergenreider E, Zeiher AM, Kroll J, Fleming I, Dimmeler S MicroRNA-27a/b controls endothelial cell repulsion and angiogenesis by targeting semaphorin 6A. Blood, 2012;119(6):1607-16. 2012 [PMID: 22184411] (Western Blot, Human)
• Assou S, Le Carrour T, Tondeur S, Strom S, Gabelle A, Marty S, Nadal L, Pantesco V, Reme T, Hugnot JP, Gasca S, Hovatta O, Hamamah S, Klein B, De Vos J A meta-analysis of human embryonic stem cells transcriptome integrated into a web-based expression atlas. Stem Cells, 2007;25(4):961-73. 2007 [PMID: 17204602] (ICC, Human)

Bioinformatics

• Gene Symbol: SEMA6A
• Uniprot:
  • Q9H2E6()