Semaphorin 3B Antibody [Biotin]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Biotin

Semaphorin 3B Antibody [Biotin] Summary

Immunogen
A synthetic peptide made to an internal portion of the human SEMA3B protein sequence (between residues 100-200). [UniProt# Q13214]
Localization
Secreted. Accumulates in the endoplasmic reticulum.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SEMA3B
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
This SEMA3B antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry paraffin embedded sections. In Western Blot, a band is seen at ~50 kDa, representing the secreted form of the protein and also a faint band at ~83 kDa, representing the pro-form of the protein. In ICC/IF, strong staining of endoplasmic reticulum and lighter signal in cytoplasm was observed in neuro2a cells. In IHC-P, staining was observed secreted, in the cytoplasm, and in the endoplasmic reticulum of mouse kidney tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Readout System

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Semaphorin 3B Antibody [Biotin]

  • (semaphorin) 3B
  • LUCA-1
  • Sema A(V)
  • sema domain, immunoglobulin domain (Ig), short basic domain, secreted
  • Sema V
  • SemA
  • SEMA3B
  • SEMA5
  • SEMA5FLJ34863
  • SEMAA
  • Semaphorin 3B
  • semaphorin A
  • semaphorin V
  • semaphorin-3B
  • semaphorin-V
  • SEMAV

Background

One family of inhibitory axon guidance molecules is the semaphorins. The semaphorins include secreted, transmembrane, and GPI anchored extracellular molecules that are involved in regulating axon guidance by inhibiting axons from growing toward incorrect targets. SEMA 3B inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Semaphorin 3B Antibody (NB100-2218B) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 488 Labeled NB100-2218G
DyLight 550 Labeled NB100-2218R
DyLight 650 Labeled NB100-2218C
HRP Labeled NB100-2218H

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Bioinformatics Tool for Semaphorin 3B Antibody (NB100-2218B)

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Bioinformatics

Gene Symbol SEMA3B
Entrez