Semaphorin 3B Antibody [DyLight 405]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 405

Semaphorin 3B Antibody [DyLight 405] Summary

Immunogen
A synthetic peptide made to an internal portion of the human SEMA3B protein sequence (between residues 100-200). [UniProt# Q13214]
Localization
Secreted. Accumulates in the endoplasmic reticulum.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SEMA3B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
This SEMA3B antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry paraffin embedded sections. In Western Blot, a band is seen at ~50 kDa, representing the secreted form of the protein and also a faint band at ~83 kDa, representing the pro-form of the protein. In ICC/IF, strong staining of endoplasmic reticulum and lighter signal in cytoplasm was observed in neuro2a cells. In IHC-P, staining was observed secreted, in the cytoplasm, and in the endoplasmic reticulum of mouse kidney tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Semaphorin 3B Antibody [DyLight 405]

  • (semaphorin) 3B
  • LUCA-1
  • Sema A(V)
  • sema domain, immunoglobulin domain (Ig), short basic domain, secreted
  • Sema V
  • SemA
  • SEMA3B
  • SEMA5
  • SEMA5FLJ34863
  • SEMAA
  • Semaphorin 3B
  • semaphorin A
  • semaphorin V
  • semaphorin-3B
  • semaphorin-V
  • SEMAV

Background

SEMA3B (semaphorin-3B) belong to semaphorins family, a large class of secreted or membrane-associated proteins which act as chemotactic cues for cell migration and axon guidance during CNS development. Secreted SEMA3s are recruited to plexins by neuropilin (NP) receptors and NP1 preferentially binds to SEMA3B. Neuronal and non-neuronal tissues exhibit abundant and differential expression of SEMA3B which inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. Besides CNS, semaphorins are expressed in other tissues also wherein they implicate in organogenesis, cell survival, proliferation, apoptosis, migration, angiogenesis, immuno-regulation and tumorigenesis. SEMA3B and another secreted semaphorin SEMA3F have been isolated from a region on human chromosome 3p21.3 which is commonly found deleted in tumors. SEMA3B is either absent or expressed at very low levels in ovarian, lung, and breast cancer cells, and when its expression is restored, it inhibits tumor growth as well as angiogenesis suggesting its role as tumor suppressor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SEMA3B
Entrez