Western Blot: Semaphorin 3B Antibody [NB100-2218] - Western blot analysis of SEMA3B in A. Hela WCE B. Ntera2 C. A431 D. HepG2 E. MCF7 F. NIH/3T3 G. PC12 H. Cos7
Immunocytochemistry/ Immunofluorescence: Semaphorin 3B Antibody [NB100-2218] - SEMA3B antibody (1:50) was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and ...read more
Immunohistochemistry: Semaphorin 3B Antibody [NB100-2218] - IHC analysis of SEMA3B in mouse kidney using DAB with hematoxylin counterstain.
Immunocytochemistry/ Immunofluorescence: Semaphorin 3B Antibody [NB100-2218] - SEMA3B antibody was tested in Neuro-2a cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and ...read more
Simple Western: Semaphorin 3B Antibody [NB100-2218] - Simple Western lane view shows a specific band for SEMA3B in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Tris-glycine, 150 mM NaCl
0.05% Sodium Azide
Immunogen affinity purified
Western Blot 1:5000-1:20000
Simple Western 1:25
This SEMA3B antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry paraffin embedded sections. In Western Blot, a band is seen at ~50 kDa, representing the secreted form of the protein and also a faint band at ~83 kDa, representing the pro-form of the protein. In ICC/IF, strong staining of endoplasmic reticulum and lighter signal in cytoplasm was observed in neuro2a cells. In IHC-P, staining was observed secreted, in the cytoplasm, and in the endoplasmic reticulum of mouse kidney tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Read Publication using NB100-2218 in the following applications:
sema domain, immunoglobulin domain (Ig), short basic domain, secreted
SEMA3B (semaphorin-3B) belong to semaphorins family, a large class of secreted or membrane-associated proteins which act as chemotactic cues for cell migration and axon guidance during CNS development. Secreted SEMA3s are recruited to plexins by neuropilin (NP) receptors and NP1 preferentially binds to SEMA3B. Neuronal and non-neuronal tissues exhibit abundant and differential expression of SEMA3B which inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. Besides CNS, semaphorins are expressed in other tissues also wherein they implicate in organogenesis, cell survival, proliferation, apoptosis, migration, angiogenesis, immuno-regulation and tumorigenesis. SEMA3B and another secreted semaphorin SEMA3F have been isolated from a region on human chromosome 3p21.3 which is commonly found deleted in tumors. SEMA3B is either absent or expressed at very low levels in ovarian, lung, and breast cancer cells, and when its expression is restored, it inhibits tumor growth as well as angiogenesis suggesting its role as tumor suppressor.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for Semaphorin 3B Antibody (NB100-2218). (Showing 1 - 1 of 1 FAQ).
Can you please confirm that product catalog # NB100-2218, lot 3B is not approved to react with Hamster samples?
NB100-2218 lot 3B has not been tested for reactivity with Hamster samples and for this reason, we do not currently recommend it for use in Hamster. If you are interested in testing this antibody against Hamster, you may be interested in reviewing our Innovator's Reward Program. You may contact email@example.com for further details about the Innovators Reward Program.