Semaphorin 3B Antibody

Images

 
Western Blot: Semaphorin 3B Antibody [NB100-2218] - Western blot analysis of SEMA3B in A. Hela WCE B. Ntera2 C. A431 D. HepG2 E. MCF7 F. NIH/3T3 G. PC12 H. Cos7
Immunocytochemistry/ Immunofluorescence: Semaphorin 3B Antibody [NB100-2218] - SEMA3B antibody (1:50) was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and ...read more
Immunohistochemistry-Paraffin: Semaphorin 3B Antibody [NB100-2218] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse kidney using 1:200 dilution of rabbit anti-Semaphorin 3B antibody. This ...read more
Immunocytochemistry/ Immunofluorescence: Semaphorin 3B Antibody [NB100-2218] - SEMA3B antibody was tested in Neuro-2a cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and ...read more
Immunohistochemistry: Semaphorin 3B Antibody [NB100-2218] - IHC analysis of SEMA3B in mouse kidney using DAB with hematoxylin counterstain.
Simple Western: Semaphorin 3B Antibody [NB100-2218] - Simple Western lane view shows a specific band for SEMA3B in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, PmSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Semaphorin 3B Antibody Summary

Immunogen
A synthetic peptide made to an internal portion of the human SEMA3B protein sequence (between residues 100-200). [UniProt# Q13214]
Localization
Secreted. Accumulates in the endoplasmic reticulum.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SEMA3B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:20000
  • Simple Western 1:25
  • Immunocytochemistry/Immunofluorescence 1:40
  • Immunohistochemistry 1:100 - 1:200
  • Immunohistochemistry-Paraffin 1:100 - 1:200
Application Notes
This SEMA3B antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry paraffin embedded sections. In Western Blot, a band is seen at ~50 kDa, representing the secreted form of the protein and also a faint band at ~83 kDa, representing the pro-form of the protein. In ICC/IF, strong staining of endoplasmic reticulum and lighter signal in cytoplasm was observed in neuro2a cells. In IHC-P, staining was observed secreted, in the cytoplasm, and in the endoplasmic reticulum of mouse kidney tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Positive Control
Brain Cerebellum Lysate (NB820-59658)
Semaphorin 3B Lysate (NBL1-15798)
Publications
Read Publication using
NB100-2218 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Semaphorin 3B Antibody

  • (semaphorin) 3B
  • LUCA-1
  • Sema A(V)
  • sema domain, immunoglobulin domain (Ig), short basic domain, secreted
  • Sema V
  • SemA
  • SEMA3B
  • SEMA5
  • SEMA5FLJ34863
  • SEMAA
  • Semaphorin 3B
  • semaphorin A
  • semaphorin V
  • semaphorin-3B
  • semaphorin-V
  • SEMAV

Background

SEMA3B (semaphorin-3B) belong to semaphorins family, a large class of secreted or membrane-associated proteins which act as chemotactic cues for cell migration and axon guidance during CNS development. Secreted SEMA3s are recruited to plexins by neuropilin (NP) receptors and NP1 preferentially binds to SEMA3B. Neuronal and non-neuronal tissues exhibit abundant and differential expression of SEMA3B which inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. Besides CNS, semaphorins are expressed in other tissues also wherein they implicate in organogenesis, cell survival, proliferation, apoptosis, migration, angiogenesis, immuno-regulation and tumorigenesis. SEMA3B and another secreted semaphorin SEMA3F have been isolated from a region on human chromosome 3p21.3 which is commonly found deleted in tumors. SEMA3B is either absent or expressed at very low levels in ovarian, lung, and breast cancer cells, and when its expression is restored, it inhibits tumor growth as well as angiogenesis suggesting its role as tumor suppressor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Semaphorin 3B Antibody (NB100-2218)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


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IHC-P
(1)
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Filter By Species
Human
(1)
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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Semaphorin 3B Antibody (NB100-2218). (Showing 1 - 1 of 1 FAQ).

  1. Can you please confirm that product catalog # NB100-2218, lot 3B is not approved to react with Hamster samples?
    • NB100-2218 lot 3B has not been tested for reactivity with Hamster samples and for this reason, we do not currently recommend it for use in Hamster. If you are interested in testing this antibody against Hamster, you may be interested in reviewing our Innovator's Reward Program. You may contact innovators@novusbio.com for further details about the Innovators Reward Program.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-2218AF405
Alexa Fluor 488 Labeled NB100-2218AF488
Alexa Fluor 647 Labeled NB100-2218AF647
Alexa Fluor 700 Labeled NB100-2218AF700
Biotin Labeled NB100-2218B
DyLight 405 Labeled NB100-2218V
DyLight 488 Labeled NB100-2218G
DyLight 550 Labeled NB100-2218R
DyLight 650 Labeled NB100-2218C
FITC Labeled NB100-2218F
HRP Labeled NB100-2218H

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Bioinformatics

Gene Symbol SEMA3B
Entrez