Secretagogin Antibody (JE60-48)

Western Blot: Secretagogin Antibody (JE60-48) [NBP3-32933] - Western blot analysis of Secretagogin on different lysates with Rabbit anti-Secretagogin antibody (NBP3-32933) at 1/2,000 dilution. Lane 1: TT cell lysate Lane 2: Human brain tissue lysate Lane 3: Mouse eyeball tissue lysate Lane 4: Mouse pancreas tissue lysate Lane 5: Rat eyeball tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 32 kDa Observed band size: 32 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32933) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Secretagogin Antibody (JE60-48) [NBP3-32933] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Secretagogin antibody (NBP3-32933) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32933) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: Secretagogin Antibody (JE60-48) [NBP3-32933] - Immunocytochemistry analysis of TT cells labeling Secretagogin with Rabbit anti-Secretagogin antibody (NBP3-32933) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Secretagogin antibody (NBP3-32933) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Flow Cytometry: Secretagogin Antibody (JE60-48) [NBP3-32933] - Flow cytometric analysis of TT cells labeling Secretagogin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32933, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clone: JE60-48
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Secretagogin Antibody (JE60-48) Summary

• Description: Novus Biologicals Rabbit Secretagogin Antibody (JE60-48) (NBP3-32933) is a recombinant monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide within Human Secretagogin aa 227-276 / 276. (Uniprot: O76038)
• Localization: Cytoplasm, Cytoplasmic vesicle, Membrane, Secreted.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SCGN
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Western Blot 1:2000
• Theoretical MW: 32 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Secretagogin Antibody (JE60-48)

• CALBL
• DJ501N12.8
• SCGN
• SECRET
• Secretagogin
• secretagogin, EF-hand calcium binding protein
• SECRETsecretagogin
• SEGN
• Setagin

Background

The encoded protein is a secreted calcium-binding protein which is found in the cytoplasm. It is related to calbindin D-28K and calretinin. This protein is thought to be involved in KCL-stimulated calcium flux and cell proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SCGN