Hexosaminidase A/HEXA Antibody [Unconjugated]

Western blot shows lysate of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human Hexosaminidase A/HEXA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6237) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Hexosaminidase A/HEXA at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysate of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Hexosaminidase A/HEXA at approximately 59 kDa (as indicated) using 20 µg/mL of Sheep Anti-Human Hexosaminidase A/HEXA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6237) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Hexosaminidase A/HEXA Antibody [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Hexosaminidase A/HEXA
  Leu23-Thr529
  Accession # P06865
• Specificity: Detects human Hexosaminidase A/HEXA in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: HEXA
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Simple Western 20 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Hexosaminidase A/HEXA Antibody [Unconjugated]

• beta-hexosaminidase subunit alpha
• Beta-N-acetylhexosaminidase subunit alpha
• EC 3.2.1
• EC 3.2.1.52
• HEXA
• hexosaminidase A (alpha polypeptide)
• Hexosaminidase A
• Hexosaminidase subunit A
• MGC99608
• N-acetyl-beta-glucosaminidase subunit alpha
• TSD

Background

HEXA (beta-hexosaminidase subunit alpha; also hexaminidase subunit alpha) is a 50-56 kDa glycoprotein member of the glycosyl hydrolase #20 family of enzymes. It is found in the lysosomes of a variety of cell types including fibroblasts, lacrimal acinar cells, and neurons. beta -Hexosaminidase is a multimeric enzyme that cleaves aminoacetylhexosamines from multiple glycosylated molecules. It exists in three noncovalent isozyme forms, among which is an A form that is trimeric and composed of one alpha - and two beta -subunits, a B form that is tetrameric and contains two alpha - and two beta -subunits, and a dimeric S form that contains two alpha -subunits. The alpha - and beta -subunits are products of related but distinct genes. The beta -hexosiminidase A form acts on a wide range of substrates, while the B form activity is biased towards sulfated hexoses. Human HEXA is synthesized as a 529 aa (amino acid) prepro-precursor. It contains a 22 aa signal sequence, a 66 aa prosegment, and a 441 aa mature region that contains a catalytic domain (aa 167-488). The prosegment may undergo additional processing through His90, and this segment (≈ 6 kDa) is known to remain linked to the mature region via a disulfide bond. Notably, circulating HEXA is comprised only of subunit proforms whose MW may run some 10 kDa higher in SDS-PAGE. Over aa 23-529, human HEXA shares 85% and 57% aa sequence identity with mouse HEXA and the human HEXA beta -subunit proprecursor, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Hexosaminidase A/HEXA Antibody (AF6237)(1)

Publication using AF6237

• Aviles-Huerta, D;Del Pizzo, R;Kowar, A;Baig, AH;Palazzo, G;Stepanova, E;Amaya Ramirez, CC;D'Agostino, S;Ratto, E;Pechincha, C;Siefert, N;Engel, H;Du, S;Cadenas-De Miguel, S;Miao, B;Cruz-Vilchez, VM;Müller-Decker, K;Elia, I;Sun, C;Palm, W;Loayza-Puch, F; Dual Ribosome Profiling reveals metabolic limitations of cancer and stromal cells in the tumor microenvironment Nature communications 2025-05-19 [PMID: 40389477] (Western Blot, Human)

Bioinformatics

• Gene Symbol: HEXA
• Uniprot:
  • P06865()