SCAP Antibody

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Western Blot: SCAP Antibody [NBP2-15127] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with SCAP antibody [C2C3], C-term diluted at 1:2000. The HRP-conjugated anti-rabbit IgG ...read more
Immunohistochemistry-Paraffin: SCAP Antibody [NBP2-15127] - Mouse kidney. SREBF chaperone protein antibody [C2C3], C-term dilution: 1:500.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: SCAP Antibody [NBP2-15127] - (30 ug of whole cell lysate) Hep G2 5% SDS PAGE, antibody diluted at 1:5000.
Immunohistochemistry-Paraffin: SCAP Antibody [NBP2-15127] - Mouse kidney. SREBF chaperone protein antibody [C2C3], C-term dilution: 1:500.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, BvSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

SCAP Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human SCAP. The exact sequence is proprietary.
Predicted Species
Rat (95%), Porcine (97%), Bovine (96%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SCAP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
140 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 1
using
NBP2-15127 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for SCAP Antibody

  • KIAA0199
  • PSEC0227
  • SREBF chaperone
  • SREBP cleavage-activating protein
  • sterol regulatory element binding protein cleavage-activating protein

Background

Sterol regulatory element binding protein (SREBP) cleavage-activating protein (SCAP) is a molecular chaperone that plays a role in the biosynthesis of cholesterol and triglycerides (1). SCAP is a membrane protein that helps shuttle SREBP from the endoplasmic reticulum (ER) via coat protein II (COPII) vesicles to the golgi where SREBP is cleaved via protease S1P and S2P, becomes activated, and is translocated to the nucleus for transcription (1-3). The SCAP protein has a theoretical molecular weight of 140 kDa and is comprised of 1276 amino acids with an N-terminal domain containing 8 transmembrane alpha helices, where transmembrane 2-6 consist of a sterol-sensing domain, and a C-terminal domain with WD40 motif repeats (1,3). The SCAP-SREBP association plays a pivotal role in regulating lipid metabolism and lipogenesis (1,4). SREBP activation via SCAP is associated with accumulation of triglycerides and cholesterol leading to metabolic disorders including diabetes, atherosclerosis, and nonalcoholic fatty liver disease (NAFLD) (1,4). Animal models have revealed that inhibition or blocking of SCAP using antagonists may be a potential therapeutic target for hyperlipidemia and hypertriglyceridemia (1,4).

References

1. Lee, S. H., Lee, J. H., & Im, S. S. (2020). The cellular function of SCAP in metabolic signaling. Experimental & molecular medicine, 52(5), 724-729. https://doi.org/10.1038/s12276-020-0430-0

2. Cheng, X., Li, J., & Guo, D. (2018). SCAP/SREBPs are Central Players in Lipid Metabolism and Novel Metabolic Targets in Cancer Therapy. Current topics in medicinal chemistry, 18(6), 484-493. https://doi.org/10.2174/1568026618666180523104541

3. Brown, M. S., Radhakrishnan, A., & Goldstein, J. L. (2018). Retrospective on Cholesterol Homeostasis: The Central Role of Scap. Annual review of biochemistry, 87, 783-807. https://doi.org/10.1146/annurev-biochem-062917-011852

4. Moon Y. A. (2017). The SCAP/SREBP Pathway: A Mediator of Hepatic Steatosis. Endocrinology and metabolism (Seoul, Korea), 32(1), 6-10. https://doi.org/10.3803/EnM.2017.32.1.6

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Review for SCAP Antibody (NBP2-15127) (1) 11

Average Rating: 1
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-15127:
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Images Ratings Applications Species Date Details
Western Blot SCAP NBP2-15127
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1
reviewed by:
Xin Shen
WB Human 04/24/2017
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Summary

ApplicationWestern Blot
Sample TestedHela whole cell lysate
SpeciesHuman

Comments

CommentsExperiment condition: primary antibody 1 in 2500 diluted in 5% milk (PBST). Main band at wrong size (below 130KDa). With the old santa cruz antibody SCAP was detected at 140-150KDa.

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Secondary Antibodies

 

Isotype Controls

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Array NBP2-15127

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Research Areas for SCAP Antibody (NBP2-15127)

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Blogs on SCAP.

SREBP2 - regulating cholesterol homeostasis and lipid metabolism
Sterol regulatory element-binding proteins (SREBP) are important transcription factors regulating the synthesis and uptake of lipids including cholesterol. This essential role in lipid metabolism makes investigations into the functions SREBPs im...  Read full blog post.

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Xin Shen
04/24/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol SCAP
Uniprot