INSIG-1 Antibody - BSA Free

Western Blot: INSIG-1 Antibody [NB110-55244] - Detection of Insig 1 in HepG2 lyaste using NB110-55244.

Immunocytochemistry/Immunofluorescence: INSIG-1 Antibody [NB110-55244] - INSIG-1 antibody was tested in HepG2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

• Reactivity: Hu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.02 mg/ml

INSIG-1 Antibody - BSA Free Summary

• Immunogen: A synthetic peptide representing an internal region of the human INSIG-1 protein (between residues 1-100) according to Swiss-Prot# O15503.
• Localization: ER Membrane
• Specificity: This is specific for Insig1 but not Insig2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: INSIG1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Western Blot 2 ug/ml
• Application Notes: This INSIG-1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where a band is seen at ~30 kDa representing the Insig1 protein. In ICC/IF positive staining was localized to the ER membrane in HepG2 cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 30 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  INSIG-1 Overexpression Lysate

Reactivity Notes

Human and rat.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.1% Sodium Azide
• Concentration: 1.02 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for INSIG-1 Antibody - BSA Free

• CL6
• CL-6
• INSIG-1 membrane protein
• INSIG-1
• insulin induced gene 1
• insulin-induced gene 1 protein
• MGC1405

Background

Oxysterols regulate cholesterol homeostasis through liver X receptor (LXR) and sterol regulatory element-binding protein (SREBP) mediated signaling pathway. This gene is an insulin-induced gene. It encodes an endoplasmic reticulum (ER) membrane protein that plays a critical role in regulating cholesterol concentrations in cells. This protein binds to the sterol-sensing domains of SREBP cleavage-activating protein (SCAP) and HMG CoA reductase, and is essential for the sterol-mediated trafficking of the two proteins. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for INSIG-1 Antibody (NB110-55244)(6)

Publications using NB110-55244

• Segatto M, Di Giovanni AL, Marino M, Pallottini V. Analysis of the protein network of cholesterol homeostasis in different brain regions: an age and sex dependent perspective J Cell Physiol 2012-12-31 [PMID: 23280554] (WB, Rat)
• Segatto M, Trapani L, Lecis C, Pallottini V. Regulation of cholesterol biosynthetic pathway in different regions of the rat central nervous system. Acta Physiol (Oxf). 2012-05-16 [PMID: 22591135] (WB, Rat)
• Trapani L, Segatto M, Simeoni V et al. Short- and long-term regulation of 3-hydroxy 3-methylglutaryl coenzyme A reductase by a 4-methylcoumarin Biochimie 2011-07-01 [PMID: 21530605] (WB, Human)
• De Marinis E, Martini C, Trentalance A et al. Sex differences in hepatic regulation of cholesterol homeostasis. J Endocrinol;198(3):635-43. 2008-09-01 [PMID: 18603607]
• Beehler K MiR-1908 Is a Cholesterol Responsive MicroRNA Implicated In Cholesterol Regulation Thesis 2020-01-01 (WB, Human)
• Nakanishi T, Tanaka R, Tonai S Et al. LH induces de novo cholesterol biosynthesis via SREBP activation in granulosa cells during ovulation in female mice Endocrinology 2021-08-25 [PMID: 34431998]

Review for INSIG-1 Antibody (NB110-55244) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 07/15/2018

Summary

• Application: Western Blot
• Sample Tested: U251 glioma cell line; whole cell lysate,U87 whole cell lysates
• Species: Human
• Lot: A

Comments

• Comments: the molecular weight of the insig1 protein in the U251 lysate is about 25-26kD。(not the 30kD indicated on the manual). The strips are clearly visible and meet the submission requirements. The dilution ratio is (2ug/ml)

Bioinformatics

• Gene Symbol: INSIG1