INSIG-1 Antibody

Images

 
Western Blot: INSIG-1 Antibody [NB110-55244] - Detection of Insig 1 in HepG2 lyaste using NB110-55244.
Immunocytochemistry/ Immunofluorescence: INSIG-1 Antibody [NB110-55244] - INSIG-1 antibody was tested in HepG2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.02 mg/ml

INSIG-1 Antibody Summary

Immunogen
A synthetic peptide representing an internal region of the human INSIG-1 protein (between residues 1-100) according to Swiss-Prot# O15503.
Localization
ER Membrane
Specificity
This is specific for Insig1 but not Insig2.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
INSIG1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Application Notes
This INSIG-1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where a band is seen at ~30 kDa representing the Insig1 protein. In ICC/IF positive staining was localized to the ER membrane in HepG2 cells.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
INSIG-1 Overexpression Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB110-55244 in the following applications:

Publications
Read Publications using
NB110-55244 in the following applications:

  • WB
    3 publications

Reactivity Notes

Human and rat.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1.02 mg/ml
Purity
Immunogen affinity purified

Alternate Names for INSIG-1 Antibody

  • CL6
  • CL-6
  • INSIG-1 membrane protein
  • INSIG-1
  • insulin induced gene 1
  • insulin-induced gene 1 protein
  • MGC1405

Background

Oxysterols regulate cholesterol homeostasis through liver X receptor (LXR) and sterol regulatory element-binding protein (SREBP) mediated signaling pathway. This gene is an insulin-induced gene. It encodes an endoplasmic reticulum (ER) membrane protein that plays a critical role in regulating cholesterol concentrations in cells. This protein binds to the sterol-sensing domains of SREBP cleavage-activating protein (SCAP) and HMG CoA reductase, and is essential for the sterol-mediated trafficking of the two proteins. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for INSIG-1 Antibody (NB110-55244)(4)

Review for INSIG-1 Antibody (NB110-55244) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB110-55244:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot INSIG-1 NB110-55244
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reviewed by:
kong yang
WB Human 07/15/2018
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Summary

ApplicationWestern Blot
Sample TestedU251 glioma cell line; whole cell lysate,U87 whole cell lysates
SpeciesHuman
LotA

Comments

Commentsthe molecular weight of the insig1 protein in the U251 lysate is about 25-26kD。(not the 30kD indicated on the manual). The strips are clearly visible and meet the submission requirements. The dilution ratio is (2ug/ml)

Product General Protocols

View specific protocols for INSIG-1 Antibody (NB110-55244): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for INSIG-1 Antibody (NB110-55244) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB110-55244AF350
Alexa Fluor 405 NB110-55244AF405
Alexa Fluor 488 NB110-55244AF488
Alexa Fluor 532 NB110-55244AF532
Alexa Fluor 594 NB110-55244AF594
Alexa Fluor 647 NB110-55244AF647
Alexa Fluor 700 NB110-55244AF700
Alexa Fluor 750 NB110-55244AF750
Biotin NB110-55244B
DyLight 350 NB110-55244UV
DyLight 405 NB110-55244V
DyLight 488 NB110-55244G
DyLight 550 NB110-55244R
DyLight 594 NB110-55244DL594
DyLight 650 NB110-55244C
DyLight 680 NB110-55244FR
DyLight 755 NB110-55244IR
FITC NB110-55244F
HRP NB110-55244H
Janelia Fluor 549 NB110-55244JF549
Janelia Fluor 646 NB110-55244JF646

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

kong yang
07/15/2018
Application: WB
Species: Human

Bioinformatics

Gene Symbol INSIG1