Western Blot: INSIG-1 Antibody [NB110-55244] - Detection of Insig 1 in HepG2 lyaste using NB110-55244.
Immunocytochemistry/ Immunofluorescence: INSIG-1 Antibody [NB110-55244] - INSIG-1 antibody was tested in HepG2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight ...read more
This INSIG-1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where a band is seen at ~30 kDa representing the Insig1 protein. In ICC/IF positive staining was localized to the ER membrane in HepG2 cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
30 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
PBS and 30% Glycerol
0.1% Sodium Azide
Immunogen affinity purified
Alternate Names for INSIG-1 Antibody
INSIG-1 membrane protein
insulin induced gene 1
insulin-induced gene 1 protein
Oxysterols regulate cholesterol homeostasis through liver X receptor (LXR) and sterol regulatory element-binding protein (SREBP) mediated signaling pathway. This gene is an insulin-induced gene. It encodes an endoplasmic reticulum (ER) membrane protein that plays a critical role in regulating cholesterol concentrations in cells. This protein binds to the sterol-sensing domains of SREBP cleavage-activating protein (SCAP) and HMG CoA reductase, and is essential for the sterol-mediated trafficking of the two proteins. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
the molecular weight of the insig1 protein in the U251 lysate is about 25-26kD。(not the 30kD indicated on the manual). The strips are clearly visible and meet the submission requirements. The dilution ratio is (2ug/ml)
Product General Protocols
View specific protocols for INSIG-1 Antibody (NB110-55244):
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