Scaffold attachment factor B2 Antibody

Western Blot: Scaffold attachment factor B2 Antibody [NB100-58791] - Detection of Human SAFB2 on HeLa whole cell lysate using NB100-58791. SAFB2 was also immunoprecipitated by rabbit anti-SAFB2 antibodies NB100-58792 and another SAFB Ab.

Immunohistochemistry: Scaffold attachment factor B2 Antibody [NB100-58791] - Sample: FFPE section of human small cell lung cancer. Antibody: Affinity purified rabbit anti- SAFB2 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Immunohistochemistry-Paraffin: Scaffold attachment factor B2 Antibody [NB100-58791] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-SAFB2 used at a dilution of 1:100.

Immunohistochemistry-Paraffin: Scaffold attachment factor B2 Antibody [NB100-58791] - FFPE section of non-small cell lung cancer. Affinity purified rabbit anti-SAFB2 used at a dilution of 1:500.

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Scaffold attachment factor B2 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 175 and 225 of human scaffold attachment factor B2 using the numbering given in entry NP_055464.1 (GeneID 9667).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SAFB2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: Western Blot - 1:2,000 to 1:10,000. Immunoprecipitation - 2 to 5 mcg/mg lysate. Immunohisto-/cytochemistry - Not tested.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Scaffold attachment factor B2 Antibody

• KIAA0138SAF-B2
• scaffold attachment factor B2

Background

Activity of the estrogen receptor (ER) is regulated through interaction with coactivators and corepressors. These proteins are present in large complexes, suggesting functional interactions among various cofactors. The scaffold attachment factor SAFB2, a component of high molecular weight nuclear protein complexes which contains a core set of protein interaction partners, functions as an estrogen receptor corepressor. It directly interacts with ER alpha repressing transcription via repression domains. Its overexpression results in inhibition of proliferation. SAFB2 is found in the cytoplasm, as well as the nucleus. Consistent with its cytoplasmic localization, there is also an interaction between SAFB2 and vinexin, a protein involved in linking signaling to the cytoskeleton.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SAFB2
• Uniprot:
  • NP_055464.1(Human)