SAFB Antibody

Images

 
Western Blot: SAFB Antibody [NB100-2593] - Detection of Human and Mouse SAFB1 on HeLa whole cell lysate using NB100-2593. SAFB1 was also immunoprecipitated by rabbit anti-SAFB1 antibody NB100-2592.
Immunohistochemistry: SAFB Antibody [NB100-2593] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- SAFB1 used at a dilution of 1:200 (1ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: SAFB Antibody [NB100-2593] - FFPE section of human breast adenocarcinoma. Affinity purified rabbit anti-SAFB1 used at a dilution of 1:250.
Immunohistochemistry-Paraffin: SAFB Antibody [NB100-2593] - FFPE section of mouse squamous cell carcinoma. Affinity purified rabbit anti-SAFB1 used at a dilution of 1:250.
Immunohistochemistry: SAFB Antibody [NB100-2593] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- SAFB1 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

SAFB Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 875 and the C-terminus (residue 915) of human Scaffold Attachment Factor B using the numbering given in entry NP_002958.2 (GeneID 6294).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SAFB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2-5 ug/mg lysate

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SAFB Antibody

  • glutathione S-transferase fusion protein
  • HAP
  • heat-shock protein (HSP27) estrogen response element and TATA box-bindingprotein
  • HETDKFZp779C1727
  • Hsp27 ERE-TATA binding protein
  • HSP27 ERE-TATA-binding protein
  • HSP27 estrogen response element-TATA box-binding protein
  • SAF-B
  • SAF-B1
  • SAFB1SAB-B1
  • scaffold attachment factor B
  • scaffold attachment factor B1

Background

This gene encodes a DNA-binding protein that has high specificity for scaffold or matrix attachment region DNA elements (S/MAR DNA). This protein is thought to be involved in attaching the base of chromatin loops to the nuclear matrix but there is conflicting evidence as to whether this protein is a component of chromatin or a nuclear matrix protein. Scaffold attachment factors are a specific subset of nuclear matrix proteins (NMP) that specifically bind to S/MAR. This encoded protein is thought to serve as a molecular base to assemble a 'transcriptosome complex' in the vicinity of actively transcribed genes. It is involved in the regulation of the heat shock protein 27 transcription and also can act as an estrogen receptor corepressor. This gene is a candidate gene for breast tumorigenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-88927
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB110-74569
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP1-43435
Species: Hu, Mu
Applications: WB, Flow
H00728695-B01P
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P
NB100-74413
Species: Hu, Mu, Rb
Applications: WB, ICC/IF, IHC, IHC-P, IP
NB600-41532
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
H00010153-B01P
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF
H00006690-M01
Species: Hu
Applications: WB, ELISA, IHC-P, IP
NB100-58791
Species: Hu
Applications: WB, IHC, IHC-P, IP
AF2685
Species: Hu
Applications: WB, Simple Western, IHC
NBP2-19533
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-46179
Species: Hu
Applications: WB, IHC, IHC-P, IP
H00000053-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P, RNAi
NBP1-86658
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
H00055835-M02
Species: Hu
Applications: WB, ELISA, IP
NBP1-82847
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-19534
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-2593
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP

Publications for SAFB Antibody (NB100-2593) (0)

There are no publications for SAFB Antibody (NB100-2593).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for SAFB Antibody (NB100-2593) (0)

There are no reviews for SAFB Antibody (NB100-2593). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SAFB Antibody (NB100-2593) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional SAFB Products

Bioinformatics Tool for SAFB Antibody (NB100-2593)

Discover related pathways, diseases and genes to SAFB Antibody (NB100-2593). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for SAFB Antibody (NB100-2593)

Discover more about diseases related to SAFB Antibody (NB100-2593).
 

Pathways for SAFB Antibody (NB100-2593)

View related products by pathway.

PTMs for SAFB Antibody (NB100-2593)

Learn more about PTMs related to SAFB Antibody (NB100-2593).

Blogs on SAFB

There are no specific blogs for SAFB, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our SAFB Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol SAFB
Entrez
Uniprot