Vinexin Antibody

Images

 
Western Blot: Vinexin Antibody [NBP2-20861] - Sample (30 ug of whole cell lysate) A: IMR32 10% SDS PAGE gel, diluted at 1:2000.
Immunocytochemistry/ Immunofluorescence: Vinexin Antibody [NBP2-20861] - Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SORBS3 protein stained by Vinexin antibody, Internal diluted at ...read more
Immunohistochemistry-Paraffin: Vinexin Antibody [NBP2-20861] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:250 dilution.

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Vinexin Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Vinexin. The exact sequence is proprietary.
Localization
Isoform Alpha: Cell junction (By similarity); Cytoplasm; cytoskeleton (By similarity); Isoform Beta: Cell junction (By similarity); Nucleus
Predicted Species
Rat (92%), Bovine (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SORBS3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for Vinexin Antibody

  • SCAM-1SH3-containing adapter molecule 1
  • SCAM1vinexin beta (SH3-containing adaptor molecule-1)
  • SH3D4VINEXIN
  • sorbin and SH3 domain containing 3
  • Sorbin and SH3 domain-containing protein 3
  • vinexin

Background

The SORBS3 gene encodes 2 isoforms vinexin-alpha and -beta that are vinculin (VCL; MIM 193065)-binding cytoskeletal proteins involved in focal adhesion and cell-cell adhesion (Kioka et al., 1999 [PubMed 9885244]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SORBS3
Uniprot