BTF Antibody

Western Blot: BTF Antibody [NBP2-24859] - Total protein from human A431 and U2Os cells and mouse 3T3 and Neuro2A cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-BTF (NBP2-24859) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume Chemiluminescent detection reagent (NBP2-61915)

Immunocytochemistry/Immunofluorescence: BTF Antibody [NBP2-24859] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-BTF at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunocytochemistry/Immunofluorescence: BTF Antibody [NBP2-24859] - NIH3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-BTF at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry-Paraffin: BTF Antibody [NBP2-24859] - analysis of human spleen probed with BTF antibody at 10 ug/ml.

Western Blot: BTF Antibody [NBP2-24859] - analysis of human BTF in Jurkat cell lysate in the 1) absence and 2) presence of immunizing peptide using BTF antibody at 0.5 ug/ml.

• Reactivity: Hu, Mu, Ca, Pm, Pm
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

BTF Antibody Summary

• Immunogen: A portion of amino acids 700-750 of human BTF was used as the immunogen for this antibody.
• Predicted Species: Chimpanzee (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BCLAF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 0.5-2 ug/ml

Reactivity Notes

The amino acid sequence used as immunogen for this antibody is 87% homologous in Orangutan.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.2 ml PBS and 0.2% Gelatin
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for BTF Antibody

• bcl-2-associated transcription factor 1
• BCL2-associated transcription factor 1
• Btf
• BTFKIAA0164bK211L9.1

Background

Btf localization is regulated by apoptotic signals, and loss of emerin binding to Btf may be relevant to muscle wasting in Emery-Dreifuss muscular dystrophy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BCLAF1
• Uniprot:
  • Q9NYF8(Human)