LRRC47 Antibody

Western Blot: LRRC47 Antibody [NBP1-93597] - Analysis using Anti-LRRC47 antibody NBP1-93597 (A) shows similar pattern to independent antibody NBP1-93598 (B).

Immunocytochemistry/Immunofluorescence: LRRC47 Antibody [NBP1-93597] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli fibrillar center & cytosol.

Immunohistochemistry-Paraffin: LRRC47 Antibody [NBP1-93597] - Staining of human cerebral cortex.

Western Blot: LRRC47 Antibody [NBP1-93597] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunohistochemistry-Paraffin: LRRC47 Antibody [NBP1-93597] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: LRRC47 Antibody [NBP1-93597] - Staining of human cerebral cortex, colon, kidney and testis using Anti-LRRC47 antibody NBP1-93597 (A) shows similar protein distribution across tissues to independent antibody NBP1-93598 (B).

Immunohistochemistry-Paraffin: LRRC47 Antibody [NBP1-93597] - Staining of human testis.

Immunohistochemistry-Paraffin: LRRC47 Antibody [NBP1-93597] - Staining of human kidney.

Immunohistochemistry-Paraffin: LRRC47 Antibody [NBP1-93597] - Staining of human colon.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

LRRC47 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: PGNALKRFLTSQTKLHEDLCEKRTAATLATHELRAVKGPLLYCARPPQDLKIVPLGRKEAKAKELVRQLQLEAEEQRKQKKRQSVSGLHRYLHLLDGNENYPCLVDADGDVISFPPITNSEK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LRRC47
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  LRRC47 Protein (NBP1-93597PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for LRRC47 Antibody

• KIAA1185
• leucine rich repeat containing 47
• leucine-rich repeat-containing protein 47

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LRRC47