SASH1 Antibody - BSA Free

Immunohistochemistry: SASH1 Antibody [NBP1-26651] - Sections of human breast carcinoma (left) and lung carcinoma (right). Antibody: Affinity purified rabbit anti- SASH1 used at a dilution of 1:1,000 (1ug/ml) and 1:400 (2.5 ug/ml). Detection: DAB and Red-fluorescent goat anti-rabbit IgG-heavy and lightchain, cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100.

Immunoprecipitation: SASH1 Antibody [NBP1-26651] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit used for IP at 3 mcg/mg lysate. SASH1 was also immunoprecipitated by rabbit anti- SASH1 antibody NBP1-26650, which recognizes an upstream epitope.

• Reactivity: Hu
• Applications: ICC/IF, IHC, IP, WB (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

SASH1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SASH1 Antibody - BSA Free (NBP1-26651) is a polyclonal antibody validated for use in IHC, ICC/IF and IP. Anti-SASH1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 750 and 800 of human SAM and SH3 domain containing 1 using the numbering given in entry NP_056093.3 (GeneID 23328).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SASH1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:250-1:1000
  • Immunohistochemistry 1:500-1:2000
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 3-5 ug/mg lysate
• Application Notes: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SASH1 Antibody - BSA Free

• dJ323M4
• dJ323M4.1
• KIAA0790RP3-323M4.1
• PEPE1
• Proline-glutamate repeat-containing protein
• SAM and SH3 domain containing 1,2500002E12Rik
• SAM and SH3 domain-containing protein 1
• SH3D6A

Background

SASH1 (SAM and SH3 domain containing 1) was identified in an in silico analysis of loss of heterozygosity (LOH) to identify genes downregulated in breast cancer. Since this discovery, it has also been found to be a candidate tumor suppressor in colon cancer. Structurally, SASH1 contains two SAM (sterile alpha motif) domains and one SH3 domain indicating a role in signal transduction and as a signaling adapter.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SASH1 Antibody (NBP1-26651)(2)

Publications using NBP1-26651

• Zhou N, Liu C, Wang X et al. Downregulated SASH1 Expression Indicates Poor Clinical Prognosis in Gastric Cancer Hum. Pathol. 2018-01-07 [PMID: 29320755] (IHC-P, Human)
• Weidmann H, Touat-Hamici Z, Durand H et al. SASH1, a new potential link between smoking and atherosclerosis. Atherosclerosis. [PMID: 26318107] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: SASH1
• Entrez:
  • 23328(Human)
• Uniprot:
  • NP_056093.3(Human)