Lipolysis Stimulated Lipoprotein Receptor Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Staining in human stomach and kidney tissues . Corresponding LSR RNA-seq data are presented ...read more
Western Blot: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Analysis in human cell lines A-431 and U-251MG using anti-LSR antibody. Corresponding LSR RNA-seq data are presented for the same cell ...read more
Immunocytochemistry/ Immunofluorescence: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & cytosol. Antibody ...read more
Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Staining of human endometrium shows strong membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Staining of human kidney shows weak positivity in cells in tubules.
Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Staining of human liver shows moderate membranous positivity in hepatocytes.
Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Staining of human placenta shows strong membranous positivity in trophoblast.
Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Staining of human stomach shows strong membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Staining of human testis shows moderate membranous and cytoplasmic positivity in cells in seminiferous ducts.
Simple Western: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Simple Western lane view shows a specific band for LSR in 0.2 mg/ml of RT-4 (left), A431 (right) lysate. This experiment was performed ...read more
Simple Western: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Electropherogram image(s) of corresponding Simple Western lane view. Lipolysis Stimulated Lipoprotein Receptor antibody was used at 1:20 ...read more

Product Details

Summary
Reactivity Hu, Mu, Po, RMSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

Lipolysis Stimulated Lipoprotein Receptor Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: MSEVTSLHEDDWRSRPSRGPALTPIRDEEWGGHSPRSPRGWDQEPAREQAGGGWRARRPRARSVDALDDLTPPSTAESGSRSPTSNGGRSRAYMPPRSRSRDDLYDQDDS
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LSR
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20-1:50
  • Immunohistochemistry-Paraffin 1:20-1:50
  • Knockdown Validated
  • Simple Western 1:20
  • Western Blot 0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue, internal validation. Use in KD reported in scientific publication (PMID: 32423802).
Control Peptide
Lipolysis Stimulated Lipoprotein Receptor Protein (NBP1-89631PEP)
Publications
Read Publications using
NBP1-89631 in the following applications:

  • 2 publications
  • 1 publication
  • KD
    1 publication
  • WB
    5 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25753034). Primate (Rhesus macaques) reported in scientific literature (PMID: 26067369). Porcine reactivity reported in scientific literature (PMID: 24048008)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Lipolysis Stimulated Lipoprotein Receptor Antibody

  • ILDR3
  • immunoglobulin-like domain containing receptor 3
  • lipolysis stimulated lipoprotein receptor
  • lipolysis-stimulated lipoprotein receptor
  • lipolysis-stimulated receptor
  • lipolysis-stimulated remnant
  • LISCH protein
  • LISCH
  • LISCH7
  • liver-specific bHLH-Zip transcription factor
  • LSR
  • MGC10659
  • MGC48312
  • MGC48503

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Lipolysis Stimulated Lipoprotein Receptor Antibody (NBP1-89631)(12)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC/IF, IHC-P, KD, WB.


Filter By Application
ICC/IF
(2)
IHC-P
(1)
KD
(1)
WB
(5)
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Filter By Species
Human
(4)
Mouse
(2)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NBP1-89631 Applications Species
Ohwada K, Konno T, Kohno T Et al. Effects of HMGB1 on Tricellular Tight Junctions via TGF-beta Signaling in Human Nasal Epithelial Cells International journal of molecular sciences Aug 4 2021 [PMID: 34445093]
Kohno T, Konno T, Kikuchi S Et al. Translocation of LSR from tricellular corners causes macropinocytosis at cell-cell interface as a trigger for breaking out of contact inhibition FASEB journal : official publication of the Federation of American Societies for Experimental Biology Sep 1 2021 [PMID: 34403506] (WB) WB
Kodera Y, Kohno T, Konno T et al. HMGB1 enhances epithelial permeability via p63/TGF-beta signaling in lung and terminal bronchial epithelial cells Tissue Barriers Aug 28 2020 [PMID: 32857676]
Kodera Y, Chiba H, Konno T et al. HMGB1-downregulated angulin-1/LSR induces epithelial barrier disruption via claudin-2 and cellular metabolism via AMPK in airway epithelial Calu-3 cells Biochem. Biophys. Res. Commun. Jun 25 2020 [PMID: 32423802] (ICC/IF, WB, KD, Human) ICC/IF, WB, KD Human
Konno T, Kohno T, Kikuchi S et al. Epithelial barrier dysfunction and cell migration induction via JNK/cofilin/actin by angubindin-1 Tissue Barriers Nov 29 2019 [PMID: 31782346]
Konno T, Kohno T, Kikuchi S et al. Localization of Tricellular Tight Junction Molecule LSR at Midbody and Centrosome During Cytokinesis in Human Epithelial Cells J. Histochem. Cytochem. Oct 29 2019 [PMID: 31662022]
Konno T, Takano K, Kaneko Y et al. Guanylate binding protein-1-mediated epithelial barrier in human salivary gland duct epithelium. Exp. Cell Res. Jul 22 2018 [PMID: 30044945] (IHC-P, Human) IHC-P Human
SHIMADA H, SATOHISA S, kOHNO T et al. Downregulation of lipolysis‑stimulated lipoprotein receptor promotes cell invasion via claudin‑1‑mediated matrix metalloproteinases in human endometrial cancer. Oncology letters. [PMID: 29151917] (WB, Human) WB Human
Takano K, Kakuki T, Kaneko Y et al. Histone deacetylase inhibition prevents cell death induced by loss of tricellular tight junction proteins in temperature-sensitive mouse cochlear cells PLoS ONE 2017 Aug 02 [PMID: 28767685] (WB, Mouse) WB Mouse
Miyata R, Kakuki T, Nomura K et al. Poly(I:C) induced microRNA-146a regulates epithelial barrier and secretion of proinflammatory cytokines in human nasal epithelial cells. Eur. J. Pharmacol. 2015 May 07 [PMID: 25959385] (WB, Human) WB Human
Show All 12 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Lipolysis Stimulated Lipoprotein Receptor Antibody (NBP1-89631). (Showing 1 - 1 of 1 FAQs).

  1. In the datasheet of your antibody against the Lipolysis-stimulated lipoprotein receptor (NBP1-89631) you show a figure in which a immunohistochemistry staining is shown. Can you please give an explanation why the cytoplasm of the cells is stained? Because
    • Lipolysis Stimulated Lipoprotein Receptor/LSR is a 649 amino acids long (71.4 kDa) trans-membrane protein with Extracellular (aa 1-259), Helical (260-280) and Cytoplasmic (281 – 649) domains Uniprot Site . Please note that NBP1-89631 was generated against a peptide corresponding to aa 431-540 of human LSR, and this sequence falls within cytoplasmic domain of LSR protein. Accordingly, NBP1-89631 is expected to bind the cytoplasmic domain of LSR protein and in immunostaining assays, the expected staining pattern with this antibody would be cytoplasmic as shown in the validation image of its datasheet.

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Bioinformatics

Gene Symbol LSR