Lipolysis Stimulated Lipoprotein Receptor Antibody

Images

 
Orthogonal Strategies: Western Blot: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Analysis in human cell lines A-431 and U-251MG using anti-LSR antibody. Corresponding LSR RNA-seq data are ...read more
Immunocytochemistry/ Immunofluorescence: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & ...read more
Orthogonal Strategies: Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Staining in human stomach and kidney tissues. Corresponding LSR RNA-seq data are presented ...read more
Western Blot: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Analysis in human cell line CACO-2.
Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Staining of human endometrium shows strong membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Staining of human kidney shows weak positivity in cells in tubules.
Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Staining of human liver shows moderate membranous positivity in hepatocytes.
Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Staining of human placenta shows strong membranous positivity in trophoblast.
Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Staining of human stomach shows strong membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Staining of human testis shows moderate membranous and cytoplasmic positivity in cells in seminiferous ducts.
Simple Western: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Simple Western lane view shows a specific band for LSR in 0.2 mg/ml of RT-4 (left), A431 (right) lysate. This experiment was performed ...read more
Simple Western: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Electropherogram image(s) of corresponding Simple Western lane view. Lipolysis Stimulated Lipoprotein Receptor antibody was used at 1:20 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

Lipolysis Stimulated Lipoprotein Receptor Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: MSEVTSLHEDDWRSRPSRGPALTPIRDEEWGGHSPRSPRGWDQEPAREQAGGGWRARRPRARSVDALDDLTPPSTAESGSRSPTSNGGRSRAYMPPRSRSRDDLYDQDDS
Specificity
Specificity of human Lipolysis Stimulated Lipoprotein Receptor antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LSR
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 0.4 ug/ml
  • Simple Western 1:20
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control Peptide
Lipolysis Stimulated Lipoprotein Receptor Protein (NBP1-89631PEP)
Publications
Read Publications using
NBP1-89631 in the following applications:

Reactivity Notes

Reactivity reported in scientific literature (PMID: 24637461)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Lipolysis Stimulated Lipoprotein Receptor Antibody

  • ILDR3
  • immunoglobulin-like domain containing receptor 3
  • lipolysis stimulated lipoprotein receptor
  • lipolysis-stimulated lipoprotein receptor
  • lipolysis-stimulated receptor
  • lipolysis-stimulated remnant
  • LISCH protein
  • LISCH
  • LISCH7
  • liver-specific bHLH-Zip transcription factor
  • MGC10659
  • MGC48312
  • MGC48503

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Lipolysis Stimulated Lipoprotein Receptor Antibody (NBP1-89631)(4)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Lipolysis Stimulated Lipoprotein Receptor Antibody (NBP1-89631). (Showing 1 - 1 of 1 FAQs).

  1. In the datasheet of your antibody against the Lipolysis-stimulated lipoprotein receptor (NBP1-89631) you show a figure in which a immunohistochemistry staining is shown. Can you please give an explanation why the cytoplasm of the cells is stained? Because
    • Lipolysis Stimulated Lipoprotein Receptor/LSR is a 649 amino acids long (71.4 kDa) trans-membrane protein with Extracellular (aa 1-259), Helical (260-280) and Cytoplasmic (281 – 649) domains Uniprot Site. Please note that NBP1-89631 was generated against a peptide corresponding to aa 431-540 of human LSR, and this sequence falls within cytoplasmic domain of LSR protein. Accordingly, NBP1-89631 is expected to bind the cytoplasmic domain of LSR protein and in immunostaining assays, the expected staining pattern with this antibody would be cytoplasmic as shown in the validation image of its datasheet.

Secondary Antibodies

 

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Diseases for Lipolysis Stimulated Lipoprotein Receptor Antibody (NBP1-89631)

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Bioinformatics

Gene Symbol LSR