SARM1 Antibody (HL1070) - Azide and BSA Free

Western Blot: SARM1 Antibody (HL1070) [NBP3-13710] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SARM1 antibody [HL1070] (NBP3-13710) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: SARM1 Antibody (HL1070) [NBP3-13710] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SARM1 antibody [HL1070] (NBP3-13710) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

ICC/IF: SARM1 Antibody (HL1070) [NBP3-13710] - SH-SY-5Y cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SARM1 stained by SARM1 antibody [HL1070] diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.Blue: Fluoroshield with DAPI.

Western Blot: SARM1 Antibody (HL1070) [NBP3-13710] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SARM1 antibody [HL1070] (NBP3-13710) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

SARM1 antibody [HL1070] detects SARM1 protein by immunofluorescent analysis.Sample: SH-SY-5Y cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SARM1 stained by SARM1 antibody [HL1070] (NBP3-13710) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SARM1 antibody [HL1070] (NBP3-13710) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: HL1070
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

SARM1 Antibody (HL1070) - Azide and BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein encompassing a sequence within the center region of human SARM1. The exact sequence is proprietary.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SARM1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Assay dependent
  • Western Blot 1:500-1:3000
• Theoretical MW: 79 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for SARM1 Antibody (HL1070) - Azide and BSA Free

• KIAA0524SAM domain-containing protein 2
• SAMD2
• SAMD2sterile alpha and HEAT/Armadillo motif protein, ortholog of Drosophila
• SARM1
• SARMFLJ36296
• Sterile alpha and Armadillo repeat protein
• sterile alpha and TIR motif containing 1
• sterile alpha and TIR motif-containing protein 1
• Sterile alpha motif domain-containing protein 2
• Tir-1 homolog

Background

Toll-like receptors (TLRs) play an essential role in the detection and elimination of invading microbes. They are type-1 transmembrane receptors, containing extracellular leucine rich repeats and an intracellular Toll/interleukin-1 receptor (TIR) domain. Upon stimulation, these receptors interact with specific TIR domain-containing adaptor proteins. Five such adaptors have been discovered to date, MyD88, Mal (MyD88 adaptor-like)/TIRAP (TIR domain-containing adaptor protein), Trif (TIR-domain-containing adaptor inducing interferon-beta), TRAM (Trif-related adaptor molecule) and SARM (SAM and ARM-containing protein). Different TLRs use different combinations of these adaptors, leading to the activation of common and unique pathways involved in the elimination of the invading microbe.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SARM1