S100A7/Psoriasin Antibody

Western Blot: S100A7/Psoriasin Antibody [NB100-40802] - HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human S100A7 with C-terminal MYC tag probed with (0.5ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with (1mg/ml) in Lane B.

Immunohistochemistry-Paraffin: S100A7/Psoriasin Antibody [NB100-40802] - Staining of Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Western Blot: S100A7/Psoriasin Antibody [NB100-40802] - Analysis of human skin lysate (35ug protein in RIPA buffer) using psoriasin antibody at 0.1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Product Discontinued

S100A7/Psoriasin Antibody Summary

• Immunogen: Peptide with sequence C-HKYTRRDDKIDKP corresponding to internal region according to NP_002954.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: S100A7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 15 kDa band observed in human skin lysates (calculated MW of 11.5 kDa band according to NP_002954.1). In transfected HEK293 transiently expressing full-length human S100A7 (myc and DYKDDDDK tagged), a band of approx. 16 kDa band was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. approx. 15 kDa band was also observed in human skin lysates (calculated MW of 11.5 kDa band according to NP_002954.1).
  IHC: Paraffin embedded Human Tonsil.
• Control:

  S100A7/Psoriasin Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for S100A7/Psoriasin Antibody

• HID 5
• HID5
• HID-5
• protein S100-A7
• PSOR1
• psoriasin 1
• Psoriasin
• S100 calcium binding protein A7 (psoriasin 1)
• S100 calcium binding protein A7
• S100 calcium-binding protein A7 (psoriasin 1)
• S100 calcium-binding protein A7
• S100A7
• S100A7c

Background

Psoriasin / S100A7 is encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein differs from the other S100 proteins of known structure in its lack of calcium binding ability in one EF-hand at the N-terminus. This protein is markedly over-expressed in the skin lesions of psoriatic patients, but is excluded as a candidate gene for familial psoriasis susceptibility. The exact function of this protein is not known.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for S100A7/Psoriasin Antibody (NB100-40802)(1)

Publication using NB100-40802

• Webb M, Emberley ED, Lizardo M et al. Expression analysis of the mouse S100A7/psoriasin gene in skin inflammation and mammary tumorigenesis. BMC Cancer 2005-02-17 [PMID: 15717926]

Bioinformatics

• Gene Symbol: S100A7
• Entrez:
  • 6278(Human)
• Uniprot:
  • NP_002954.1(Human)