RUNX1/CBFA2 Antibody (6C12A5) - BSA Free

Western Blot: RUNX1/CBFA2 Antibody (6C12A5) [NBP2-61861] - Analysis using RUNX1 mAb against human RUNX1 (AA: 237-337) recombinant protein. (Expected MW is 36.9 kDa)

Flow Cytometry: RUNX1/CBFA2 Antibody (6C12A5) [NBP2-61861] - Analysis of K562 cells using RUNX1 mouse mAb (green) and negative control (red).

Western Blot: RUNX1/CBFA2 Antibody (6C12A5) [NBP2-61861] - Analysis using RUNX1 mAb against HEK293 (1) and RUNX1 (AA: 237-337)-hIgGFc transfected HEK293 (2) cell lysate.

ELISA: RUNX1/CBFA2 Antibody (6C12A5) [NBP2-61861] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

• Reactivity: Hu
• Applications: WB, ELISA, Flow
• Clone: 6C12A5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

RUNX1/CBFA2 Antibody (6C12A5) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human RUNX1/CBFA2 (AA: 237-337) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RUNX1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:200-1:400
  • Western Blot 1:500-1-2000
• Theoretical MW: 48.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for RUNX1/CBFA2 Antibody (6C12A5) - BSA Free

• Acute myeloid leukemia 1 protein
• AML1
• AML1acute myeloid leukemia 1
• AML1-EVI-1
• CBFA2
• CBFA2acute myeloid leukemia 1 protein (oncogene AML-1), core-binding factor, alphasubunit10AMLCR1
• Core-binding factor subunit alpha-2
• EVI-1
• PEBP2A2
• runt domain, alpha subunit 2
• runt-related transcription factor 1
• RUNX1
• SL3/AKV core-binding factor alpha B subunit

Background

Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. The protein encoded by this gene represents the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis. Chromosomal translocations involving this gene are well-documented and have been associated with several types of leukemia. Three transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RUNX1