RPTOR Antibody

Images

 
Western Blot: RPTOR Antibody [NB100-766] - Detection of Human Raptor by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit ...read more
Flow Cytometry: RPTOR Antibody [NB100-766] - Flow cytometric detection of Raptor protein. 1 million Jurkat cells were fixed, permeabilized, and stained with 4.0 ug/ml anti-Raptor NB100-766 in a 100 ul reaction. ...read more
Immunoprecipitation: RPTOR Antibody [NB100-766] - Immunoprecipitation of Human Raptor. Samples: Whole cell lysate (1 mg/IP, 1/4 of IP loaded) from HeLa cells. Antibody: Affinity purified rabbit anti-Raptor antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
   

Biological Strategies

     

Order Details

RPTOR Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human Regulatory Associated Protein of mTOR using the numbering given in entry AAM09075.1 (GeneID 57521).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RPTOR
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000 -1:25000
  • Flow Cytometry 5 ug per 1 million cells in a 150 mcl reaction
  • Immunoprecipitation 2 -5 ug/mg lysate
Application Notes
This antibody may be useful for Flow Cytometry 5 ug per 1 million cells in a 150 mcl reaction.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RPTOR Antibody

  • KIAA1303
  • KIAA1303p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats
  • KOG1
  • Mip1
  • p150 target of rapamycin (TOR)-scaffold protein
  • Raptor
  • regulatory associated protein of MTOR, complex 1
  • regulatory-associated protein of mTOR
  • RPTOR

Background

Raptor (regulatory associated protein of mTOR) is a 150 kDa mTOR (Target of Rapamycin) binding protein. It plays a positive role in nutrient-stimulated signaling and cell size maintenance.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol RPTOR
Entrez
Uniprot