RPE65 Antibody (401.8B11.3D9) [Alexa Fluor® 488]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Ch, XpSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
401.8B11.3D9
Clonality
Monoclonal
Host
Mouse

RPE65 Antibody (401.8B11.3D9) [Alexa Fluor® 488] Summary

Immunogen
Bovine RPE65 microsomal membrane proteins. [UniProt# Q28175]
Localization
Cytoplasmic, Microsomeal and Cell Membrane
Marker
Retinal Pigment Epithelium Marker
Specificity
NB100-355 is specific for RPE65.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
RPE65
Purity
Protein G purified
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Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Conjugate
Alexa Fluor 488
Format
Alexa Fluor 488 (A=488, E=515-545)
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
Immunohistochemistry and western blot. By Western blot, this RPE65 antibody recognizes a band at ~65 kDa, representing bovine RPE65 protein. It has also been tested on recombinant human protein in COS7 cells. It may also be used in immunohistochemistry-Frozen, on murine retinal sections and ICC in porcine. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Based on immunogen sequence similarity, this antibody is predicted to react with Duck/Ostrich and several other Birds species (89%), Zebrafish (74%) and Sea lamprey (71%).

Notes

Alexa Flour (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. This product is provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Alternate Names for RPE65 Antibody (401.8B11.3D9) [Alexa Fluor® 488]

  • All-trans-retinyl-palmitate hydrolase
  • EC 3.1.1.64
  • EC 5.2.1.7
  • LCA2p63
  • mRPE65
  • RBP-binding membrane protein
  • rd12
  • Retinal pigment epithelium-specific 65 kDa protein
  • retinal pigment epithelium-specific protein (65kD)
  • retinal pigment epithelium-specific protein 65kDa
  • retinitis pigmentosa 20 (autosomal recessive)
  • retinoid isomerohydrolase
  • Retinol isomerase
  • RP20
  • sRPE65

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for RPE65 Antibody (NB100-355AF488) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 647 Labeled NB100-355AF647
Alexa Fluor 700 Labeled NB100-355AF700

Additional RPE65 Products

RPE65 NB100-355AF488

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Research Areas for RPE65 Antibody (NB100-355AF488)

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Blogs on RPE65.

Using RPE65 as a tool to investigate ocular gene therapies
While not life threatening, blindness and retinal disease are profoundly debilitating and greatly affect quality of life.  Understandably, gene therapy has been subject to controversy given it’s potential effects on the rest of our cellular...  Read full blog post.

RPE65: Vision, Blindness and Hope
Retinal pigment epithelium-specific 65 kDa protein (RPE65) is an essential vision protein, and so mutations in the RPE65 gene cause blindness. However, clinical trials using gene therapy to treat patients with a defective RPE65 gene suggest that some...  Read full blog post.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol RPE65

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