RPE65 Antibody (401.8B11.3D9) [DyLight 650]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Ch, XpSpecies Glossary
Applications WB, Flow, ICC/IF, IHC-Fr, IHC-P
Clone
401.8B11.3D9
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 650

RPE65 Antibody (401.8B11.3D9) [DyLight 650] Summary

Immunogen
Bovine RPE65 microsomal membrane proteins. [UniProt# Q28175]
Localization
Cytoplasmic, Microsomeal and Cell Membrane
Marker
Retinal Pigment Epithelium Marker
Specificity
NB100-355 is specific for RPE65.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
RPE65
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
Immunohistochemistry and western blot. By Western blot, this RPE65 antibody recognizes a band at ~65 kDa, representing bovine RPE65 protein. It has also been tested on recombinant human protein in COS7 cells. It may also be used in immunohistochemistry-Frozen, on murine retinal sections and ICC in porcine. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Based on immunogen sequence similarity, this antibody is predicted to react with Duck/Ostrich and several other Birds species (89%), Zebrafish (74%) and Sea lamprey (71%).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for RPE65 Antibody (401.8B11.3D9) [DyLight 650]

  • All-trans-retinyl-palmitate hydrolase
  • EC 3.1.1.64
  • EC 5.2.1.7
  • LCA2p63
  • mRPE65
  • RBP-binding membrane protein
  • rd12
  • Retinal pigment epithelium-specific 65 kDa protein
  • retinal pigment epithelium-specific protein (65kD)
  • retinal pigment epithelium-specific protein 65kDa
  • retinitis pigmentosa 20 (autosomal recessive)
  • retinoid isomerohydrolase
  • Retinol isomerase
  • RP20
  • sRPE65

Background

Highly conserved among vertebrate species, RPE65 is a major protein of the retinal pigment epithelium (RPE). RPE65 is essential for the regeneration of rhodopsin in the visual cycle. Mutations in RPE65 are responsible for certain forms of autosomal recessive severe retinal dystrophy, including Leber Congenital Amaurosis (LCA). Loss of function results in a block in retinoid processing required for the synthesis of 11-cis retinal and the accumulation of retinyl esters in the RPE.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for RPE65 Antibody (NB100-355C). (Showing 1 - 1 of 1 FAQ).

  1. I have a customer wanting to know if there is any data that we can send them for your prod #NB100-355C RPE65 Antibody Dylight 655.
    • NB100-355C is the same as NB100-355 it is just conjugated. You can send them the data on our website for NB100-355. NB100-355C should behave similarly.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NB100-355APC
Biotin Labeled NB100-355B
DyLight 350 Labeled NB100-355UV
DyLight 405 Labeled NB100-355V
DyLight 488 Labeled NB100-355G
DyLight 550 Labeled NB100-355R
DyLight 680 Labeled NB100-355FR
DyLight 755 Labeled NB100-355IR
FITC Labeled NB100-355F
HRP Labeled NB100-355H
PE Labeled NB100-355PE
PerCP Labeled NB100-355PCP

Additional RPE65 Products

RPE65 NB100-355C

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Blogs on RPE65.

Using RPE65 as a tool to investigate ocular gene therapies
While not life threatening, blindness and retinal disease are profoundly debilitating and greatly affect quality of life.  Understandably, gene therapy has been subject to controversy given it’s potential effects on the rest of our cellular...  Read full blog post.

RPE65: Vision, Blindness and Hope
Retinal pigment epithelium-specific 65 kDa protein (RPE65) is an essential vision protein, and so mutations in the RPE65 gene cause blindness. However, clinical trials using gene therapy to treat patients with a defective RPE65 gene suggest that some...  Read full blog post.

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Bioinformatics

Gene Symbol RPE65

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