Mouse IgG1 Isotype Control (11711) [Alexa Fluor® 488]

Images

 
BG01V human embryonic stem cells were stained with MouseAnti-Human Alkaline Phosphatase/ALPL Alexa Fluor® 488‑conjugatedMonoclonal Antibody (Catalog # FAB1448G,filled histogram) or Mouse IgG Alexa Fluor® ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Ctrl
Clone
11711
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 488

Mouse IgG1 Isotype Control (11711) [Alexa Fluor® 488] Summary

Immunogen
KLH
Specificity
Selected for use as mouse IgG1 isotype control for flow cytometry applications.
Source
N/A
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
IGHG1

Applications/Dilutions

Dilutions
  • Control 5 uL/10^6 cells
Reviewed Applications
Read 3 Reviews rated 5
using
IC002G in the following application:

Publications
Read Publications using
IC002G in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse IgG1 Isotype Control (11711) [Alexa Fluor® 488]

  • IgG1

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Publications for IgG1 Isotype Control (IC002G)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Flow Cytometry, Flow Cytometry Control.


Filter By Application
Flow Cytometry
(3)
Flow Cytometry Control
(2)
All Applications
Filter By Species
Human
(5)
All Species

Reviews for IgG1 Isotype Control (IC002G) (3) 53

Average Rating: 5
(Based on 3 reviews)
We have 3 review tested in 1 species: Human.

Reviews using IC002G:
Filter by Applications
ICC/IF
(1)
Flow
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry/Immunofluorescence IgG1 IC002G
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reviewed by:
Anonymous
ICC/IF 02/06/2018
View

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Lotabv10415051

Comments

CommentsUsed as a isotype control for a dog Immunophenotyping. the dog cells were washed and incubated with a cocktail of antibodies, this antibody was used as a isotope control for the experiment.
Serum IgG1 IC002G
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reviewed by:
Anonymous
02/06/2018
View

Summary

ApplicationSerum
Lotabv10415051

Comments

CommentsUsed as a isotype control for a dog Immunophenotyping. the dog cells were washed and incubated with a cocktail of antibodies, this antibody was used as a isotope control for the experiment.
Flow Cytometry IgG1 IC002G
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reviewed by:
Anonymous
Flow Human 03/15/2016
View

Summary

ApplicationFlow Cytometry
Sample Testedhuman HEK293T cells
SpeciesHuman
LotABVI0312031

Blocking

Blocking Details2%FBS in PBS for 20 min on ice

Primary Anitbody

Dilution Ratio1:100 diluted, 1 million cells incubted with antibody in 100ul on ice for 30 min in dark

Details

Detection Notesread by flow cytometry
Fixation Details1 million cells were detached by EDTA, washed with 2%FBS in PBS
Permeabilization Detailsno permeabilization steps
Wash Description2%FBS in PBS once

Comments

CommentsIgG1 Isotype Control (IC002G) provides very low background in flow reading. It is a great antibody for isotype control purpose. I tested several cell lines, the FL1H values are only several hundreds. Five-star!

FAQs for IgG1 Isotype Control (IC002G) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

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Bioinformatics Tool for IgG1 Isotype Control (IC002G)

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Recent Reviews

5
5
3
4
0
3
0
2
0
1
0

Anonymous
02/06/2018
Application: ICC/IF
Species:

Anonymous
02/06/2018
Application:
Species:

Anonymous
03/15/2016
Application: Flow
Species: Human

Bioinformatics

Gene Symbol IGHG1
COVID-19 update