RPA70 Antibody (4C4)

Images

 
Western Blot: RPA70 Antibody (4C4) [NBP2-37500] - Western blot analysis using RPA1 mouse mAb against HeLa (1), MCF-7 (2), K562(3), A431(4), and COS-7 (6) cell lysate.
Immunocytochemistry/ Immunofluorescence: RPA70 Antibody (4C4) [NBP2-37500] - Immunofluorescence analysis of HeLa cells using RPA1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry: RPA70 Antibody (4C4) [NBP2-37500] - Immunohistochemical analysis of paraffin-embedded prostate tissues using RPA1 mouse mAb with DAB staining.
Flow Cytometry: RPA70 Antibody (4C4) [NBP2-37500] - Flow cytometric analysis of Jurkat cells using RPA1 mouse mAb (green) and negative control (red).
Western Blot: RPA70 Antibody (4C4) [NBP2-37500] - Western blot analysis using RPA1 mAb against human RPA1 (AA: 308-513) recombinant protein. (Expected MW is 48.3 kDa)
Immunohistochemistry: RPA70 Antibody (4C4) [NBP2-37500] - Immunohistochemical analysis of paraffin-embedded colon cancer tissues using RPA1 mouse mAb with DAB staining.
ELISA: RPA70 Antibody (4C4) [NBP2-37500] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
Clone
4C4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

RPA70 Antibody (4C4) Summary

Immunogen
Purified recombinant fragment of human RPA1 expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RPA1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • CyTOF-ready
Application Notes
This antibody is Cytof ready.
Theoretical MW
68.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for RPA70 Antibody (4C4)

  • HSSB
  • MST075
  • MSTP075
  • REPA1RF-A protein 1
  • Replication factor A protein 1
  • replication protein A 70 kDa DNA-binding subunit
  • replication protein A1 (70kD)
  • replication protein A1, 70kDa
  • RF-A
  • RP-A
  • RPA70RP-A p70
  • Single-stranded DNA-binding protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol RPA1