ROR gamma/RORC/NR1F3 Antibody (4G419) - BSA Free

Western Blot: ROR gamma/RORC/NR1F3 Antibody (4G419) [NBP2-24449] - Analysis in (A) human thymus lysate, (B) transfected 293 cell lysate, and (C) untransfected 293 cell lysate. Dilution: 5 ug/ml.

Flow Cytometry: ROR gamma/RORC/NR1F3 Antibody (4G419) [NBP2-24449] - Analysis using the FITC conjugate of NBP2-24449. Staining of ROR gamma in human lymphocytes using 10 ul (0.25 ug) of ROR gamma antibody. Shaded histogram represents cells without antibody; green represents isotype control antibody; red represents ROR gamma

Flow Cytometry: ROR gamma/RORC/NR1F3 Antibody (4G419) [NBP2-24449] - Intracellular analysis of ROR gamma in human lymphocytes using 0.25 ug of this antibody. Shaded histogram represents cells without antibody; green represents isotype control antibody ; red represents ROR gamma antibody.

Flow Cytometry: ROR gamma/RORC/NR1F3 Antibody (4G419) [NBP2-24449] - Intracellular flow cytometric analysis of ROR gamma in human monocytes using 0.25 ug of ROR gamma antibody. Shaded histogram represents cells without antibody; green represents isotype control antibody (Novus, 20109); red represents ROR gamma antibody.

Flow Cytometry: ROR gamma/RORC/NR1F3 Antibody (4G419) [NBP2-24449] - Analysis using the Allophycocyanin conjugate of NBP2-24449. Staining of ROR gamma in human lymphocytes using 10 ul (0.25 ug) of ROR gamma antibody. Shaded histogram represents cells without antibody; green represents isotype control antibodywas used for this test.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, Flow
• Clone: 4G419
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

ROR gamma/RORC/NR1F3 Antibody (4G419) - BSA Free Summary

• Immunogen: A portion of amino acids 1-50 of human ROR gamma was used as the immunogen.
• Predicted Species: Primate (96%). Backed by our 100% Guarantee.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RORC
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25-0.5 ug/10^6 cells
  • Western Blot 5 ug/ml

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 22110656).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for ROR gamma/RORC/NR1F3 Antibody (4G419) - BSA Free

• MGC129539
• mouse ror gamma
• NR1F3
• Nuclear receptor ROR gamma
• Nuclear receptor ROR-gamma
• Nuclear receptor RZR gamma
• Nuclear receptor RZR-gamma
• Nuclear receptor subfamily 1 group F member 3
• RAR-Related Orphan Nuclear Receptor Variant 2
• RAR-related orphan receptor C
• RAR-related orphan receptor gamma
• retinoic acid-binding receptor gamma
• retinoid-related orphan receptor gamma
• Retinoid-related orphan receptor-gamma
• ROR gamma
• RORC
• RORG
• RORGMGC129539
• RZRG
• RZRGRZR-GAMMA
• TOR

Background

ROR gamma/RORC/NR1F3, aka RAR Orphan Receptor gamma, is a Nuclear Receptor 1 Thyroid Hormone-Like receptor containing a short A-T rich sequence follow by a single core motif half-site 5'-AGGTCA-3'. RORC is a key regulator of thymopoiesis, bone metabolism, T-cell apoptosis, and lymphoid organogenesis. NR1F3 regulates intrinsic transcriptional functions. Unlike other major nuclear receptors, RORC binds as a monomer to hormone response elements and is documented in mouse thymus, adipose, bone, skeletal muscle, liver, kidney, and human skeletal muscle. An N-terminal isoform of ROR gamma, ROR gamma T, inhibits Fas ligand expression and cytokine secretion in immature thymocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ROR gamma/RORC/NR1F3 Antibody (NBP2-24449)(5)

Publications using NBP2-24449

• Jang, S;Kim, JY;Kim, CW;Kim, I; Hypertonic Salt Solution Enhances Inflammatory Responses in Cultured Splenic T-Cells from Dahl Salt-Sensitive Rats but Not Dahl Salt-Resistant Rats Journal of cardiovascular development and disease 2023-10-02 [PMID: 37887861]
• Wang T, Qiao H, Zhai Z et al. Plumbagin Ameliorates Collagen-Induced Arthritis by Regulating Treg/Th17 Cell Imbalances and Suppressing Osteoclastogenesis Front Immunol Jan 8 2018 [PMID: 30671063] (IHC, Rat)
• Cortes-Perez NG, Lozano-Ojalvo D, Maiga MA et al. Intragastric administration of Lactobacillus casei BL23 induces regulatory FoxP3+RORyt+ T cells subset in mice Benef Microbes 2017-05-30 [PMID: 28504580] (FLOW, Mouse)
  
  Details:
  This reference used the FITC version of NBP2-24449.
• Lenoir M, Del Carmen S, Cortes-Perez NG et al. Lactobacillus casei BL23 regulates Treg and Th17 T-cell populations and reduces DMH-associated colorectal cancer. J. Gastroenterol. 2016-01-09 [PMID: 26749362] (FLOW, Mouse)
• Almolda B, Costa M, Montoya M, Gonzalez B, Castellano B Increase in Th17 and T-reg lymphocytes and decrease of IL22 correlate with the recovery phase of acute EAE in rat. PLoS One. 2011-01-01 [PMID: 22110656] (FLOW, Rat)

FAQs for ROR gamma/RORC/NR1F3 Antibody (NBP2-24449). (Showing 1 - 4 of 4 FAQs).

1. Does ROR gamma/RORC/NR1F3 antibodies comes in lyophilized form?
   • Yes, we have 3 RORC antibodies delivere in lyophilized form: MAB61091, MAB61092, MAB6109.
2. What the theoretical molecular weight for ROR gamma/RORC/NR1F3 antibodies?
   • The TMW of ROR gamma/RORC/NR1F3 antibodies is approximately 56 - 60.
3. Since the protocol to isolate cells from mice seems to be quite long I would like to know if you have experience in staining with Foxp3 and Ror gamma and leaving cells overnight? Would you suggest leaving cells overnight in Fix/Perm or better after having completed the staining? And in which buffer would you leave them then?
   • Where as FOXP3 and ROR gamma staining can be stopped after the fixation step, we suggest that researchers strictly follow the protocol without variation (even if it takes a long time), when trying for the 1st time. After you establish this for your system, then you can work on optimizing a varied protocol according to your needs and convenience. In this case, after the fix/perm step (Step 13 of NBP2-266911) wash the cells in 1X Staining buffer (2 times) and suspend in Staining buffer (500 ul) overnight. Then, in the morning proceed to Step 14 and continue the protocol.
4. What is the immunogen sequence of this ROR gamma/RORC/NR1F3 antibody?
   • A sequence from amino acids 1 - 50 of Human ROR gamma.

Bioinformatics

• Gene Symbol: RORC