ROR gamma/RORC/NR1F3 Antibody (4G419) [PE]

Flow Cytometry: ROR gamma/RORC/NR1F3 Antibody (4G419) [PE] [NBP2-24452] - Intracellular analysis of RORgamma in human lymphocytes using 10 ul (0.25 ug) of ROR gamma antibody. Shaded histogram represents cells without antibody; green represents isotype control antibody this antibody ; red represents RORgamma antibody. this antibody was used for this test.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: Flow
• Clone: 4G419
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: PE

ROR gamma/RORC/NR1F3 Antibody (4G419) [PE] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: A portion of amino acids 1-50 of human ROR gamma was used as the immunogen.
• Predicted Species: Primate (96%). Backed by our 100% Guarantee.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RORC
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1ul/1 million cells
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NBP2-24449. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 22110656).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for ROR gamma/RORC/NR1F3 Antibody (4G419) [PE]

• MGC129539
• NR1F3
• Nuclear receptor ROR gamma
• Nuclear receptor ROR-gamma
• Nuclear receptor RZR gamma
• Nuclear receptor RZR-gamma
• Nuclear receptor subfamily 1 group F member 3
• RAR-Related Orphan Nuclear Receptor Variant 2
• RAR-related orphan receptor C
• RAR-related orphan receptor gamma
• retinoic acid-binding receptor gamma
• retinoid-related orphan receptor gamma
• Retinoid-related orphan receptor-gamma
• ROR gamma
• RORC
• RORG
• RORGMGC129539
• RZRG
• RZRGRZR-GAMMA
• TOR

Background

ROR gamma/RORC/NR1F3, aka RAR Orphan Receptor gamma, is a Nuclear Receptor 1 Thyroid Hormone-Like receptor containing a short A-T rich sequence follow by a single core motif half-site 5'-AGGTCA-3'. RORC is a key regulator of thymopoiesis, bone metabolism, T-cell apoptosis, and lymphoid organogenesis. NR1F3 regulates intrinsic transcriptional functions. Unlike other major nuclear receptors, RORC binds as a monomer to hormone response elements and is documented in mouse thymus, adipose, bone, skeletal muscle, liver, kidney, and human skeletal muscle. An N-terminal isoform of ROR gamma, ROR gamma T, inhibits Fas ligand expression and cytokine secretion in immature thymocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ROR gamma/RORC/NR1F3 Antibody (NBP2-24452)(1)

Publication using NBP2-24452

• Almolda B, Costa M, Montoya M, Gonzalez B, Castellano B Increase in Th17 and T-reg lymphocytes and decrease of IL22 correlate with the recovery phase of acute EAE in rat. PLoS One. 2011-01-01 [PMID: 22110656] (Rat)

FAQs for ROR gamma/RORC/NR1F3 Antibody (NBP2-24452). (Showing 1 - 5 of 5 FAQ).

1. Does ROR gamma/RORC/NR1F3 antibodies comes in lyophilized form?
   • Yes, we have 3 RORC antibodies delivere in lyophilized form: MAB61091, MAB61092, MAB6109.
2. What the theoretical molecular weight for ROR gamma/RORC/NR1F3 antibodies?
   • The TMW of ROR gamma/RORC/NR1F3 antibodies is approximately 56 - 60.
3. What is the immunogen sequence of this ROR gamma/RORC/NR1F3 antibody?
   • A sequence from amino acids 1 - 50 of Human ROR gamma.
4. Please can you confirm the concentration and volume for a 100 test unit of NBP2-24452 lot number A-2-031516-F.
   • The concentration for our product NBP2-24452 Lot: A-2-031516-F is 1.0mg/ml.
5. Please can you confirm the concentration and volume for a 100 test unit of NBP2-24452 lot number A-2-031516-F.
   • The concentration for our product NBP2-24452 Lot: A-2-031516-F is 1.0mg/ml.

Bioinformatics

• Gene Symbol: RORC