RNMT Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: RNMT Antibody [NBP1-80841] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

RNMT Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: NENTTASGTGLSEKTSVCRQVDIARKRKEFEDDLVKESSSCGKDTPSKKRKLDPEIVPEEKDCGDAEGNSKKRKRETEDVPKDKSSTGDGTQNK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RNMT
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  RNMT Protein (NBP1-80841PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for RNMT Antibody - BSA Free

• DKFZp686H1252
• EC 2.1.1.56
• hcm1p
• hCMT1
• hCMT1c
• hMet
• KIAA0398
• mRNA (guanine-7-)methyltransferase
• mRNA (guanine-N(7)-)-methyltransferase
• mRNA cap guanine-N7 methyltransferase
• mRNA cap methyltransferase
• RG7MT1MET
• RNA (guanine-7-) methyltransferase

Background

mRNA cap methyltransferase (HCM) catalyzes the N7 methylation of the cap formed by mRNA capping enzyme (HCE) to form a terminal 7-methylguanosine. This is subsequently recognised by cap binding proteins that facilitate key events in gene expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RNMT Antibody (NBP1-80841)(2)

Publications using NBP1-80841

• Conti G, Calderan L, Quintero Sierra LA et al. Tumor and peritumoral adipose tissue crosstalk: de-differentiated adipocytes influence spread of colon carcinoma cells Tissue and Cell 2022-12-01 [PMID: 36542947] (WB, Mouse)
• Wang Q, Wehbe A, Wills M et al. The Key Role of Initiation Timing on Stroke Rehabilitation by Remote Ischemic Conditioning with Exercise (RICE) Neurological research 2022-11-18 [PMID: 36399507]

Bioinformatics

• Gene Symbol: RNMT
• Entrez:
  • 8731(Human)
• Uniprot:
  • O43148(Human)