![Western Blot: RNA Polymerase II/POLR2A [p Ser7] Antibody [NBP3-16906] - Western blot analysis of extracts of MCF7 cells, using RNA Polymerase II/POLR2A antibody (NBP3-16906) at 1:1000 dilution.MCF7 cells were treated by Doxorubicin (0.5 uM) at 37C for 24 hours.MCF7 cells were treated by nocodazole (50 ng/ml) at 37C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/RNA-Polymerase-II-POLR2A-[p-Ser7]-Antibody-Western-Blot-NBP3-16906-img0001.jpg "Western Blot: RNA Polymerase II/POLR2A [p Ser7] Antibody [NBP3-16906] - Western blot analysis of extracts of MCF7 cells, using RNA Polymerase II/POLR2A antibody (NBP3-16906) at 1:1000 dilution.MCF7 cells were treated by Doxorubicin (0.5 uM) at 37C for 24 hours.MCF7 cells were treated by nocodazole (50 ng/ml) at 37C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ChIP, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Description | Novus Biologicals Rabbit RNA Polymerase II/POLR2A [p Ser7] Antibody - Azide and BSA Free (NBP3-16906) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | A phospho specific peptide corresponding to residues surrounding S7 of human RNA Polymerase II/POLR2A. SPSYS |
| Modification | p Ser7 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | POLR2A |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 217 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 50% glycerol |
| Preservative | 0.05% Proclin 300 |
| Purity | Affinity purified |
![RNA Polymerase II/POLR2A [p Ser7] Antibody - Azide and BSA Free](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "RNA Polymerase II/POLR2A [p Ser7] Antibody - Azide and BSA Free")
Secondary Antibodies |
Isotype Controls |
Research Areas for RNA Polymerase II/POLR2A Antibody (NBP3-16906)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | POLR2A |
![Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser7] Antibody - Azide and BSA Free [NBP3-16906] - Immunohistochemistry analysis of paraffin-embedded Rat brain using RNA Polymerase II/POLR2A Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16906_rabbit-rna-polymerase-ii-polr2a-p-ser-7-pab-52202511361258.jpg "Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser7] Antibody - Azide and BSA Free [NBP3-16906] - Immunohistochemistry analysis of paraffin-embedded Rat brain using RNA Polymerase II/POLR2A Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser7] Antibody - Azide and BSA Free [NBP3-16906] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using RNA Polymerase II/POLR2A Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16906_rabbit-rna-polymerase-ii-polr2a-p-ser-7-pab-52202511365198.jpg "Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser7] Antibody - Azide and BSA Free [NBP3-16906] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using RNA Polymerase II/POLR2A Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser7] Antibody - Azide and BSA Free [NBP3-16906] - Immunohistochemistry analysis of paraffin-embedded Mouse brain using RNA Polymerase II/POLR2A Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16906_rabbit-rna-polymerase-ii-polr2a-p-ser-7-pab-52202511374167.jpg "Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser7] Antibody - Azide and BSA Free [NBP3-16906] - Immunohistochemistry analysis of paraffin-embedded Mouse brain using RNA Polymerase II/POLR2A Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Chromatin Immunoprecipitation: RNA Polymerase II/POLR2A [p Ser7] Antibody - Azide and BSA Free [NBP3-16906] - Chromatin immunoprecipitation was performed with cross-linked chromatin from HeLa cells, using RNA Polymerase II/POLR2A Rabbit pAb and rabbit IgG . The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram compares the ratio of the immunoprecipitated DNA versus the input.](http://images.novusbio.com/fullsize/nbp3-16906_rabbit-rna-polymerase-ii-polr2a-p-ser-7-pab-52202511402732.jpg "Chromatin Immunoprecipitation: RNA Polymerase II/POLR2A [p Ser7] Antibody - Azide and BSA Free [NBP3-16906] - Chromatin immunoprecipitation was performed with cross-linked chromatin from HeLa cells, using RNA Polymerase II/POLR2A Rabbit pAb and rabbit IgG . The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram compares the ratio of the immunoprecipitated DNA versus the input.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")