RIP140 Antibody

Western Blot: RIP140 Antibody [NB110-5122] - Analysis of recombinant human.

• Reactivity: Hu
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

RIP140 Antibody Summary

• Description: Novus Biologicals Rabbit RIP140 Antibody (NB110-5122) is a polyclonal antibody validated for use in WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide corresponding to residues G(718) N P T K G R V K K K E K T P L R D(735) of human RIP140.
• Specificity: Detects a band at approximately 140 kDa.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NRIP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 2 ug/ml
• Application Notes: ICC/IF usage reported in scientific literature (PMID: 7641693).
• Theoretical MW: 140 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Cross-reactivity with Non-human primate.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RIP140 Antibody

• 10RIP140Receptor-interacting protein 140
• FLJ77253
• nuclear receptor interacting protein 1
• nuclear receptor-interacting protein 1
• receptor interacting protein 140

Background

Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate. Receptor Interacting Protein 140 (RIP 140) has been shown to enhance estrogen receptor transcriptional activity through the AF2 activation domain in an agonist dependent manner and has also been implicated to be involved in other steroid receptor pathways, including the retinoid pathway. RIP 140 has recently been shown to interact with peroxisome proliferator-activated receptor-alpha (PPAR alpha) and has been proposed to competitively inhibit PPAR alpha interaction with Steroid Receptor Coactivator-1 (SRC-1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for RIP140 Antibody (NB110-5122) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 09/05/2014

Summary

• Application: Western Blot
• Sample Tested: 293T cell lysate
• Species: Human

Bioinformatics

• Gene Symbol: NRIP1
• Entrez:
  • 8204(Human)